...keywords close enough... If in the past you have tried to combine multiple terms in an Addgene plasmid ...plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...Availability to Industry: Restrict search to plasmids which can be distributed to industry research scientists... GCaMP containing plasmids are available for distribution. If your experiment involves delivering a calcium...

... brain tumor biology. When not snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar...DEMONSTRATE GENOME EDITING BY THIS TOOL This post was contributed by guest blogger Pooran Dewari. Any views in ...performs Sanger sequencing on select regions of all distributed plasmids as part of quality control, but does...with the NgAgo system indicates that it is very tricky to use and fussy to optimize. One reason could ...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...enzyme." DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development...

...undergo stringent quality control by Addgene or a trusted quality control partner before distribution. Details...Production Antibody Production Many antibodies distributed by Addgene have been produced in-house by Addgene...contacting [email protected] . Some of the antibodies distributed by Addgene were developed by the Institute for..., antibodies undergo a buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered.... Only antibody samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes...

...fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays are able to recognize...zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer, cutting...their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions... targets are confined to sequences composed of triplets with corresponding zinc fingers, potential targetable...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...single antibody signal. Here, HRP is used as a colorimetric, in which the reaction produces a colored product...the amount of enzyme present in each well. In colorimetric assays, protein amount can be determined by ..., lyse, and then collect protein extract by centrifugation or affinity columns. Mix samples with a primary...immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule may be a...

...Addgene distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as ...NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from...that are currently in our repository. AAV are distributed as 100 µL of purified viral particles in solution...

...subjected to quality control by Addgene before being distributed to customers. Details about our production protocols...control are described below. AAV Production AAV distributed by Addgene has been produced either in-house ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...

...populations can arise very quickly following the tried and true process of evolution. Watch this video ...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in...resistant to antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally...resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species’ resistance...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...be unharmed by the compound it produces (think intrinsic resistance), but surrounding microbes would be...resistance genes survive and multiply. Factors contributing to the development of resistance include (Figure...

...cells to let the virions out. We recommend using a Tris-based lysis buffer and sonication.  The obtained...density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin,..., and the two helper plasmids, commonly called “triple plasmid transfection” (Figure 1).    Figure 1:...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

... be a shortage of amusing reports where someone tries to “optimize” a particular standard lab protocol...technical knowledge and judgement. For particularly tricky cases, team discussions can often lead to otherwise...

...     Over the last decade, my peers and I have tried to move away from calling non-academic careers and...hands-on lab opportunities. You can get your hands in nitrile gloves again! The opportunities are there once ...

... glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine is very effective at disrupting...and blue triangles. 2) An antibody immobilized on a bead binds specifically to the blue triangle protein...not the other. 3) The beads are collected by centrifugation or a magnet. 4) Unbound proteins are washed...target protein. The beads can be collected by centrifugation (agarose beads) or a magnet (magnetic beads...particularly advantageous because they do not require centrifugation between washes which can cause loss of beads...containing non-ionic detergents, such as NP-40 or triton-X, to preserve native protein conformations and...proteins, including those in the nucleus, more stringent buffers may be needed. Since many factors, including...

...If you’ve heard of containerization, but haven’t tried it or are confused by the concept, don’t worry–you... developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting, or sequence...first n items. To be sure, we went through much trial and error to learn the scripting language, but our...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

...secreted version of the protein is available – these attributes have made it popular with scientists since its...enchancer regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For use as a reporter of the ability... in plants, co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 pRATIO1267 RedLuc...plants, co-expressed with Gaussia luciferase for ratiometric imaging David Nelson 212935 pGL4.84(hRlucCP/Puro...Lentiviral expression of firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral...

...101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-...lines have been deposited here with Addgene for distribution to the research community. With these plasmids...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...

...amplification Viral vector production Density centrifugation purification Addgene’s comprehensive quality... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...titration, and quality control measures. We have distributed preps around the globe and shared our well-developed...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

...candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin...stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated through both phosphorylation...ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E ...

... super-REACh via a 10 amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-6aa linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus...linker-Venus VCA Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV ...VCV Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric...

...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

...a promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...Muscle-specific expression of Cre MCK Mammalian Kahn 13763 pTriEx-HTNC Cre fused to HIV-TAT - promotes cellular uptake...puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer 68448 TOPO rtTA3-2A-Cre iRFP670, Cre, and GFP ...Luo 119063 pBac(3xP3-gTc’v; Tc-hsp68:Cre) Cre Tribolium casteneum hsp68 T. castaneum (red flour beetle...Ohlmann 120279 014LP-pAAV-ANF-657- Cre-pA Cre; for atrial-specific expression ANF AAV Wehrens 121675 pAAV-EF1a-fDIO-Cre... partnership with genOway, Addgene is able to distribute materials containing FLEx technology. Read the...