Skip to main content
Addgene
Showing: 61 - 80 of 224 results

  1. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... from the Caltech lab of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription...

  2. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...neurotransmission. These receptors can be activated by an array of different ligands, including hormones, neurotransmitters... showing no constitutive activity. There are an array of DREADDs that activate different G proteins, work...

  3. Hot Plasmids - February 2022

    Type
    Blog Post
    ...guides require two arrays for Cas9, two guides can be streamlined into a single array for Cas12a. Image...

  4. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors.... nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular...

  5. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who have had a ...offices or professional organizations can provide referrals. There are bad coaches out there, so finding a...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    ...bright and stable fluorescence of the original (Ivorra-Molla et al., 2023). They also named their variant...: https://doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold ...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... the (1) binding of calcium, (2) allosteric rearrangements of the sensor, and (3) subsequent change in... with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous calmodulin...

  9. 6 Tips for Grant Writing

    Type
    Blog Post
    ...post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No matter ...  Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University College...

  11. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...into two categories: probes and editors. A probe array may consist of at least two sensor modules targeting...via CRISPR techniques. This tool-kit includes an array of FPs as well as additional useful tags, including...

  12. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...-020-00361-z Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration...https://doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J.,...

  13. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support...References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution...

  18. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer vectors..., Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, Maouche-Chrétien L, Bernaudin F, Girot R, Dorazio...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cpf1 multiplexing approach using a single crRNA array to express up to 4 crRNAs. Zetsche et al. also suggest...Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID...

  20. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...makers have moved away from long written proposal narratives. Now they require grant seekers to use online...demand for stronger budgets with clear budget narratives, logic models, established partnerships and collaborations...
Showing: 61 - 80 of 224 results