... 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted...

... E. coli strains including the popular BL21(DE3) (Li et al., 2021). This pEcCas/pEcgRNA system works similar...–1290 . https://doi.org/10.1021/acssynbio.7b00399 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021)...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...bacterial CRISPR engineering One common way of accomplishing bacterial genome engineering is done with recombineering...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...systems is base editing. This process is generally accomplished by fusing an enzyme capable of converting one...

...nar/gkw930 Yu Y, Wu X, Guan N, Shao J, Li H, Chen Y, Ping Y, Li D, Ye H (2020) Engineering a far-red light–activated... LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control... and repeatable. Since these initial studies published in 2015, more split systems have been developed...Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems.  Listen to the anti-CRISPR podcast segment Simply expressing...

... Zhu, Z., Liu, G., Kou, L., Wang, Y., Qiu, J.-L., Li, J., & Gao, C. (2021). High-efficiency prime editing...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...

...References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control ...

...gene that kills the cells expressing it? The answer lies in special strains of E. coli that tolerate the ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...

...experiment! References Becher, Burkhard, Ari Waisman, and Li-Fan Lu. "Conditional gene-targeting in mice: problems...

...References and Resources References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007) Evolving...

...why is FLEx useful? The power of the FLEx system lies in the ability to conditionally turn off one gene...optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses for FLEx switches...

...doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich L, Li C, et al. (2018) OGT binds a conserved C-terminal...

....201409074 Tran NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal ...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted...being the source of irreproducible data (Begley & Ellis, 2012; Baker, 2015). This contributes to an enormous...Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A ...276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012) Raise standards for preclinical cancer...

...and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic... trials The CRISPR therapies don’t stop with the list above! There are many, many more in the earlier ...

...expression and purification. Li J, et al. Science. 2017. PubMed PMID: 28082411 Listen to the podcast segment...increased ECT-DNA. Diving further into TZAP function, Li and colleagues observed endogenous TZAP localization...PubMed PMID: 18094608 Listen to the podcast Segement on the pPSU Plasmids B. subtilis knockout and knockdown...recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin...2016. PubMed PMID: 27238023 Listen to the podcast segment on the B. subtilis libraries Transcription factor...Cells) acrIIA1 Listeria monocytogenes  pRAU168  No  pJH372  No  acrIIA2 Listeria monocytogenes  pRAU171...   acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes...

...range 6.0 (yellow) - 8.0 (red)). Blood pH normally lies around 7.35-7.4 so mammalian cells are sensitive...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...

... genome editing can help accomplish this goal. The power of this system lies in the ability of the endonuclease...

...Addgenies has helped me get a much better idea of what lies ahead. Thus the “Meet an Addgenie” Instagram interview...choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told...don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped me realize that is...Scientists are often under a lot of pressure to publish their research and are not so keen on sharing their...came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the most purpose at Addgene...

...Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy May... Crispresso, that adhere to the same principles listed here. It employs biological best practices. For... is generally advised, there is little detail published on how to do it best. Scientists are typically...

... CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

...become clear that the power of this editing platform lies in the diversity of nucleases and their applications...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

...hyperpolarization. PSAM ligands The advantage of PSAMs lies in their ability to be specifically and potently...19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic...