...Mustafa, M., Nik Yusof, N. K., Lee, C. K., Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015...

...bacterial species, but newer versions engineered for eukaryotic cells are available and further improvements ...Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is recruited to target...25122746 Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. ... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby..., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...

...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...Mycoplasma Our 293T cell line was obtained directly from Takara, and is routinely tested for mycoplasma contamination...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...However, approximately 40% of human (and most eukaryotic) proteins localize to chemically distinct subcellular...fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway."Biochemical and biophysical...

...interactions. What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent...May; 34(5):341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer...

...Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism, is a commonly used model organism in...double every 2 hours. Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable...

...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...https://doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. ...

...Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana... M., Gotthard, G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein...

...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest...

...Laboratory. Vancouver, BC Canada 49.2827°N 123.1207°W – Karissa Milbury I attended the march in Vancouver, BC,...science in the public sphere as often as they can. Karissa Milbury is currently a PhD candidate at the BC ...

... 33,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919...92,919 Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ...

... CKR-1, CMKAR1, CXCR1, IL8R1, IL8RBA IL8RB interleukin 8 receptor, beta CD182, CDw128b, CMKAR2, CXCR2,... (C-X-C motif) receptor 3 CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR CXCR4 chemokine (C-X-C... protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2...Smith B, Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. ...

... viral service. Retrograde construct from Alla Karpova at Janelia Research Institute....preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector... these viral preparations was performed by the Karpova lab. Results for our quality control experiments...

...mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692 pRubiC-T2A-cre mCherry...Retroviral Luikart 66693 pRubiG-T2A-cre GFP and Cre expression in newborn neurons Retroviral Luikart 67503 ...

...PXL 75349 Mammalian U6 yes, activate S. pyogenes Luikart pRubiG-T2A-Cas9 75348 Mammalian/Retroviral from...from pXL yes, activate S. pyogenes EGFP Luikart pRubiC-T2A-Cas9 75347 Mammalian/Retroviral from pXL yes,...yes, activate S. pyogenes mCherry Luikart pDECKO_mCherry 78534 Mammalian hU6 none S. pyogenes Puro, mCherry...

...contains regulatory elements. Eukaryotic Promoters Eukaryotic Transcription Eukaryotic promoters are much more...the other hand, the process in eukaryotes is much more complex. Eukaryotes require a minimum of seven transcription...actually begins. Only eukaryotes and archaea, however, contain this TATA box. Most prokaryotes contain a sequence...more complex and diverse than prokaryotic promoters. Eukaryotic promoters span a wide range of DNA sequences...binding is very different in bacteria compared to eukaryotes. In bacteria, the core RNA polymerase requires...transcription factors in order for RNA polymerase II (a eukaryote-specific RNA polymerase) to bind to a promoter...Transcription is tightly controlled in both bacteria and eukaryotes. Promoters are controlled by various DNA regulatory...

...provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein...protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR...

... School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan Rego, Addgene...Campus Kenton Woodard Penn Vector Core Melina Fan, Karen Guerin, and Leila Haery from Addgene will also be...

...Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex ...