We narrowed to 298 results for: IRES

...select first hires who are well-suited, productive, and fit well with your goals. These first hires will set...set the tone and atmosphere that future hires will look up to." She also recommends to seek out advice...lab and selectively picking the first couple of hires to build a strong, collaborative team. And take ...

...three lab heads how they go about selecting new hires. This is followed by some perhaps surprising, yet...scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from throughout the...are dedicated and interested in science. “Postdoc hires are simpler because of their documented history ...with their interests.” George Church selects new hires initially based on those who notice and embrace ...

...AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded intermediate...AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles for efficient...several hours on a heated stir plate to dissolve and requires monitoring so that the solution doesn’t get too...AAV for my cells of interest. Every batch of AAV requires its own MOI optimization to account for batch-...

...preps, lentiviral titration requires an additional couple of days, as it requires transduction of more cells...companies. Production of both AAV and lentivirus requires three plasmids — a transfer plasmid containing...baculovirus plasmid. Production of baculovirus requires only two plasmids — your transfer plasmid and ...

...seem like the easier of the two, but it actually requires more technical skill to place the needle in the...the vertebrae, so hitting the correct location requires a lot of skill and anatomical knowledge. Intrathecal... the lungs. It is an advanced technique, as it requires intubation of the mice, which can be tricky given... intranasal is easier, less invasive, and only requires a pipette. Since the substances are administered...

... of hard-to-achieve factors. For example, FRET requires that fusion proteins be in close proximity and...FRET, as the overexpression of fusion proteins it requires can lead to aberrant subcellular localization ... construction Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably...information, constructing appropriate controls may requires that you empirically test a number of mutants ...

...conjugate, and changing your reporter molecule requires either re-conjugating or re-purchasing your primary...most protein from your gel to your membrane. It requires anywhere from sixty minutes to overnight to run... can be done.  Note that each type of transfer requires different equipment. Wet transfers can be done...compatible with chemiluminescence reporters and requires access to a darkroom and a developer. Film is ...exposures for a blot with varying signals on it. It requires long exposure times (up to thirty minutes!) to...

...myristoylation requires an N-terminal consensus sequence (MG-) and prenylation requires a C-terminal consensus... 2002). GFP needs oxygen Chromophore formation requires a fully folded beta-barrel structure, followed...chromophore (Tsien, 1998). Importantly, this reaction requires molecular oxygen and as a consequence GFP remains...

...mutations. Ultimately, successful allelic exchange requires two sequential homologous recombination events...events, but because these events are rare, each requires selection. These two steps are (Figure 1): The ...and excision of the vector backbone. This step requires “counter-selection,” meaning that the selection...generate precise, unmarked mutants, but it frequently requires considerable optimization to ensure strong counter-selection...

...James M. Wilson AV-9-PV0109 105533-AAV9 pAAV.CMV.Luc.IRES.EGFP.SV40 James M. Wilson AV-9-PV1262 Plasmid not...

...promoters for transcriptional regulation, so it requires a different approach for regulating transgene ...Generating iPSCs with plasmid-based expression requires serial transfection of 1 or 2 plasmids that express... the gold standard instead. Teratoma formation requires injecting iPSCs into immunodeficient mice and ...suited for these situations. The second scenario requires higher levels of safety and as little chance of...

...must be performed after primary purification and requires a second clean up step to remove the cleaved tag...Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents insoluble.... Mammalian purification is still possible but requires optimization and often more stringent washing....

... endeavor, NEBuilder requires upfront planning. This cloning methodology requires that the fragments to...simplify this process by using NEBuilder which requires only a single-stranded synthetic oligo to generate...

...frequency. Although highly useful, this technique requires extensive sample processing to avoid disrupting...appear red. To enable this color switching, TRICK requires the following components: a GFP protein that ...caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin sequences into the ...

...simultaneously. To do so with fluorescent protein labels requires multiple fluorescent proteins whose excitation... means that the choice of fluorescent proteins requires some thought. The first step in choosing fluorescent...fluorescent proteins can be separated from each other requires knowledge of their excitation and emission spectra...

...it like winter tires in different conditions: the more flexible rubber of winter tires give a better grip...recombinant proteins for industrial or research purposes requires stable host cells (the simplest example would ...

...are a non-renewable reagent - Their production requires animal sacrifice and because they are an animal...secondary Less sensitive than HRP-RANbody staining Requires secondary antibody staining HA Tagged GFP, RFP...secondary   Less sensitive than HRP-RANbody staining Requires secondary antibody staining GFP, RFP, H2A2B ...

...of these orthologs is key: since each ortholog requires a different PAM sequence, a gRNA designed for ...color is specified by PAM sequence, each color requires a different Cas9 ortholog, and the target sequence...in combination with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce ...

..., comprised of PAS and GAF domains of iRFP713. Requires presence of heme oxygenase for chromophore formation...miRFP709 and miRFP670, pair with common PAS domain. Requires presence of heme oxygenase for chromophore formation...BiFC interaction is typically irreversible and requires some time for chromophore maturation, splitFAST...

... “first generation” CRISPR activator. While it requires a relatively simple construct, it exhibits modest...activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic Activation... to other notable CRISPR activators is that it requires a fusion protein, rather than relying on a two-component...