...model your specific disease or process of interest. Do you want to: Generate complete and permanent loss...percentage of DSBs are still repaired by NHEJ. How do you determine that your desired edit has occurred...

... by Alyssa Cecchetelli and Andrew Hempstead. What do smell, taste and sight have in common, besides being...

...Systems, Harvard University.    Which titer do people usually report? Addgene and other viral vector...values to determine accuracy and reliability. When doing this, we make a judgement call on whether the two...improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean for your experiments? Because...reordering the same virus feel free to email us and we can do our best to send you a particular lot (if we still...Figure 2). Because physical titers are used for dosing purposes in preclinical studies, it is important...each lot of AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’...

...23550 1056 GEF Fbxo8 26269 319 Gateway Return to top Do you have suggestions for other plasmids that should...

... (SGD) (Link opens in a new window) Return to top Do you have suggestions for other plasmids that should...

... genetic model organism C. elegans. Return to top Do you have suggestions for other plasmids that should...

...78. doi: 10.1038/nrd4505. PubMed PMID: 25743081 . Do you have suggestions for other plasmids that should...

...thaliana based on RNA-seq analysis. Return to top Do you have suggestions for other plasmids that should...

...transcription factor bind to a particular sequence? Do these two proteins interact? What happens when I ...of modularly assembled zinc fingers. Return to Top Do you have suggestions for other plasmids that should...

... Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple Cas9s to be used...have also developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors...

...protospacer sequence) is G, do not add another G ( e.g., sgRNA-B in Table 4 ) and do not add C to the final...

...consider. What do academic and non-academic careers in science have in common? You can do quality science... to work/life scheduling Diverse opportunities to do science related jobs that require no bench work Better...available ($)—can outsource a task someone else who can do better, no grant writing Job changes can happen regularly...

...But how do you know if a masters will lead to a career you will like? Here’s two things to do to answer...decide to earn a PhD, but if I had to do grad school over, I would just do the master’s! If you'd like more...blog Learn about career planning resources What Do I Do Now? Academic v. Non-Academic Career Decisions ...wasn’t sure a master’s was a good decision. So how do you know if you should earn a master? Here are a ...job postings on a job search website like Indeed. Do the job descriptions sound interesting? Is the work... to see how people describe their actual work. To do this, try looking at LinkedIn profiles of people ...interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is their career path...

...things I’ll be doing through the course of any given week. Knowing this, I hand write to-do lists at the...Probably the easiest way to do this is to leave your phone in your bag on “Do Not Disturb” during the times...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...Time Mangement for Scientists. Back when I used to do mammalian cell culture, I would often respond to ...complete the task When picking which tasks to actually do, you should err on the side of things that have the...likely energize you. For example, let's say you’re doing a multi-day experiment in the lab. You have two ...but you’ll have to spend the entirety of each day doing the experiment. The second way, it will take you...

...the best things you can do during your training is meet interesting people doing interesting things. I ...you want to pursue someone doing a job you wish you knew more about – how do you make a connection? An ...position at their company, do NOT call this an Informational Interview. If you do this, the connection may...graduate degree in science. I find it ironic that we do all of our training as scientists (5-12 years worth...about learning new things from other scientists doing interesting work. Consider this to be like any other...manager. They might say yes and they might say no but do not confuse networking for a job connection with ...not possible consider a phone or video call. If you do decide to use Skype, make sure to follow the rules...

...you may need to find a way to do, manage and lead all in one day.  How do you convince others (in particular...all career skills leaderhsip skills when done well? Do you just have to know it when you see it? “The Three...any stage in our careers we may be in a cycle of doing, managing or leading. All three roles are important... or finishing any project. Doers are the ones who do the work Doers focus on understanding what is expected....  Managers make sure the work gets done Managers do this by removing obstacles that stand in the way ...with other teams. While they sometimes dig in and do some of the work, they must be more focused on delegating...three roles allows you to identify what you need to do to be successful in a specific role or cycle. For...

...fairs is a great start, but you need to do more than just show up. Do your homework and know who is going ...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...end, one of the most important things that you can do now is diversify. Read on for an overview of some...Further, even for those students and researchers who do take the initiative to develop themselves, resources...difficult to identify. So what specifically should you do? What follows are just a few suggestions based on...Conferences One of the single most important things you can do to plan for your career is to expose yourself to ...to other working professionals, and one way to do this is to attend conferences. Depending upon your discipline...

... single cell genomics in zebrafish. Return to top Do you have suggestions for other plasmids that should...

...Biology. 2010 Feb;2(2):a001107. PMC PMID: 20182618 . Do you have suggestions for other plasmids that should...

... FRET) with pmVenus(L68V)-mTurquoise2 Back to Top Do you have suggestions for other plasmids that should...