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We narrowed to 773 results for: Dos

Showing: 61 - 80 of 773 results
  1. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    Blog Post
    ...thank you notes and recognition. You are why we do what we do. From all of us to all of you. Thanks for supporting...When you think of a "nonprofit" organization what do you think of? Maybe the term brings to mind a social...is sustainable. The difference is what nonprofits do with their revenue. A nonprofit organization cannot...benefiting our scientific community, what other reasons do we like being a nonprofit? One big reason is that...nonprofit company culture as well. We work every day to do a better job of enabling more scientists in labs ...
  2. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

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    Blog Post
    ...questions like “How do you start to bring people back to on-site work?” and “How do you start to expand... let me keep my toe in the water of HR work. What do you think is the most important resource that Addgene...of employees, and the research environment.   What do you hope Addgene will accomplish in the near future...expand operations a little bit?”    What are you doing to enjoy your retirement? I’m also on the advisory...
  3. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post
    ...want to access that type of material - or do what biology can do - it’s good to be able to build regulation...Addgene: How easy is it to do this now? Voigt: One of the things we’ve been doing is developing software ...Building circuits from CRISPRs Addgene: What can CRISPR do for synthetic biology? Voigt: The big promise is ... networks. Addgene: What kinds of things could we do as genetic circuits become larger and more complex... But there are a couple of problems. You couldn’t do it without repeating the same guide RNA twice. The...
  4. CRISPR Plasmids - Mammalian Expression

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    Collection
    ...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a... a wide variety of Cas-containing plasmids. Do you have suggestions for other plasmids that should be ...
  5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post
    ...dicots, not grasses and wheat, but we do work in wheat here. We do lots of work on wheat rusts and we are...made the most sense to us to enable other people to do that too. We don’t have the capacity to send tools...have comparable expression levels to things people do use a lot. The problem in plant science is there ...good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those looking to...expression construct on the same piece of DNA. We do a lot of protein-protein interaction work and we’...is an analysis of women in faculty, how they are doing. They look at structure, impediments, the representation...work in plant science. I think their chances of doing something amazing will be equally high as for those...
  6. Finding Your Perfect Job After University

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    Blog Post
    ...parasitology but another major factor held me back; I do not have a second language. During the recession ...these positions.  I had to reassess what I wanted to do and so I applied for a role in Industry. I wanted...wanted to get "a real job" and roles in Industry do tend to pay better. I worked in a Microbiology testing...and spreadsheets. It was great to be in the lab doing experiments  without the constant fear of failure...focus always seemed more on getting results than doing good science. Another problem was that the work ...monotonous very quickly; as soon as I learned how to do the different tests and became efficient, I then ... had to look at what type of work I would like to do. I had taken advanced virology classes in California...
  7. Networking Follow-up: I Met a Person. Now What?

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    Blog Post
    ...relationships with people you care about knowing. How do you follow up with new connections and build the ... note about where you met and what you discussed. Do this in a timely manner (within a few days or a week... Here are a few things you probably don’t want to do: Don’t connect via LinkedIn with strangers. It’s... that don’t respond; focus on the nice people who do respond. Don’t call or email multiple times if you...They now message me frequently and ask me how I’m doing with nothing else to talk about. I know absolutely...worth connecting with people you don’t like or who do not show interest in you. Network with the people...
  8. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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    Blog Post
    ...collection. While most days I was up to my neck in to-dos, I also found time to go out to sushi restaurants... building strong connections with coworkers and doing something meaningful with my career. I can’t ...as much of her focus is on the scientific work we do while mine is mainly within content and business.... who are interested in understanding the work you do, in addition to being genuinely interested in knowing...safety net and trampoline all at once. I was able to do something that was challenging while helping us share...media spreadsheets every day of my co-op. I had to do research on loads of different best practices, create...
  9. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    Blog Post
    ...contracting life where you do a little bit of work for one client and then switch to doing something else. You...always loved science, but didn’t know what she would do if she wasn’t at the bench.  “I was literally sitting...with a postdoc, and I’m like, “Oh my god, what can I do?! This is a mistake! Why did I even come [to grad...’s a science writer?” Learning about SciComm by doing SciComm Writing had always been a strength of Susan...s something that good storytellers in science can do.” Susan’s number one piece of advice for getting...on things.” Susan personally found this easier to do while still in school since opportunities, such as...
  10. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post
    ...improve their current job situation. What can a coach do that is different from an adviser, boss or mentor...have seen, without exception, that this helps people do a better job at reaching their goals.  Check out ...aware of the benefits of coaching relationships and do not know where to look for a coach. Many (most?) ...will cost a fortune for a successful outcome. How do I find a coach? Start with referrals. Are there scientists...what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional presence...need to choose well. What are their qualifications? Do they have certifications? How can they demonstrate...and Anthony Collmann of White Consulting frequently do sessions by Skype or phone. Samantha Sutton is another...
  11. An Inside Look into Addgene's Dev Team

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    Blog Post
    ...supposed to be doing. When this happens, we discuss and agree on a common name. While we do our best to ... another point of collaboration for us. I enjoy doing code reviews because it’s like a little peek into...and fixing bugs: we take time during each sprint to do some maintenance work on our code. One way to look...is tidying up your home. You could choose to only do the dishes once a month, resulting in a dangerous...dangerous mountain of dirty dishes or you could choose to do the dishes after every meal and be reassured that...total of two years, from planning to completion, to do this upgrade while continuing to add new features...Conclusion While none of us are life science majors, we do our best to understand the science. By understanding...
  12. Lab to Office Culture Shock

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    Blog Post
    ...tools to help you do your job as well as possible. It’s in their best interest to do so, sure, but it ... something to do and there is no thumb twiddling. But you like the downtime, you say? Do you know what... anymore. Being a scientist is not about what you do--it’s about how you think. I have not worked at the... how much of that time you’re actually spending doing work. Yes, you may legitimately need to be in the...the opportunity to learn their “language”, and in doing so you may discover that your career options are...the meetings you’re attending are a waste of time, do what you can to fix them, and take a look at these...
  13. Career Insights: Technical Support Specialist

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    Blog Post
    ... issue, you will do well in a technical support role. What general characteristics do jobs in technical...Additional Resources on the Addgene Blog Read What Do I Do Now? Academic v. Non-Academic Career Decisions ...results, answer loads of questions, and I also had to do some troubleshooting as advised by the technical ...that rather unpleasant experience I thought: I could do this job so much better. Driven by that, I successfully...different from those specified on its data sheet?” or “Do you have additional data regarding the specificity... Outreach Scientist at Addgene. These days he's doing a lot of science communication, and is particularly...
  14. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post
    ...company culture! Addgene is the best place to work, we do SO many fun things!!”  Okay - honestly, maybe not...thoroughly. It’s kind of a chicken and egg situation- do we work so hard so we have time to play later? Or...how everyone is kind and collaborative and all we do is have fun all day long, whistling while we work...included) that plan big company events and fun things to do throughout the month. Although we are busy with many...tough to come up with new and interesting things to do, feel free to crowdsource! 2. Free food generally...dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary environment Team...your office space too Balance point number 3 with doing fun things within your office. This way people don...
  15. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...
  16. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post
    ...reading email). My advice is always "don't do it unless you can do it well" – even the smallest program. I... personal rule is that I do not let the panelists introduce themselves. I do a 1-2 minute introduction... with a few questions. Who is out there? What do they do? Find out their areas of science or expertise...bios were likely part of the publicity. Good panels do not waste time on long introductions. The moderator...
  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post
    ...flowers that change color throughout the day) and doing this with very little money. So when did inspiration...biology PhD’s. I was facing the prospect of having to do a third postdoc in order to rebuild my resume, so...we spent a lot of time talking about what we could do to make ourselves marketable to the wider world and...altogether. We had a lot of ideas about things we could do for work, and they weren’t restricted to science ...some point, our ideas got pretty good. We started doing extracurricular research on some of them to see ...along the lines of “I hate GMOs, but what you are doing makes sense. I can support that.” That was interesting...building this company, and that’s what we want it to do. It’s exhilarating to have that sort of freedom. ...
  18. Interview with Bart Newland, Addgene’s Newest Board Member

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    Blog Post
    ...organization and see how I can help. Q: What do you enjoy doing outside of work? BN: I love to travel and...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts...bring to Addgene -- that comfort with saying, “if we do this, then what?” I hope to bring that business experience...
Showing: 61 - 80 of 773 results