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We narrowed to 1,046 results for: sta

Showing: 761 - 780 of 1046 results

  1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types...of a single heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed...GFP regulation to other types of proteins. They started with another biological workhorse, Cre recombinase...

  2. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... the F1 generation, your breeding scheme should start with parental mice that are each homozygous for ...chromosomes in the parental generation, they are likely to stay on separate chromosomes in future generations as...homozygous for both mutations. The shorter the distance between the two loci of interest on the chromosome...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...elegans. To study the role of 3’UTRs, the Arizona State University (ASU) Biodesign institute developed a...opportunities to develop computational methods to better understand animal development (Kyoda et al., 2012).  C. ...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    ...cell cycle attempt to understand development, regeneration, and certain disease states. However, it’s difficult... each tool expanding our ability to study and understand the complex interworking of the 86 billion neurons...

  5. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ..., even if he or she is a difficult person: 1) Understand your supervisor’s expectations Every supervisor... details of your work. Describe the problem by stating the facts and explain how it is interfering with...is miscommunication. For example, you might misunderstand your supervisor’s suggestions and take your ...

  6. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral vectors are derived...Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ...lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...

  8. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...similar regions. This combination of chewing up one stand and cutting the template strand causes large deletions... to studying Cas3 and its Cascade partners to understand it and use it to its full potential. Hopefully...Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R....

  9. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...questions. Practice your presentation for both instances. If your poster material is nearing publication... this is being used, it’s best to turn to an established social media platform. During the conference ...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...

  10. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic...definitive, but visual identification is a good place to start before taking on more time consuming and expensive...use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used codon optimization programs...

  11. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degradation of the tagged protein. Degrons are destabilizing domains which will be identified by various ...protein must be expressed (inducibly, ectopically, stably… it’s your call). The TIR1 F-box protein will associate.... If your project also involves imaging at some stage, then choosing a HaloTag could pay dividends down...

  12. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...cloning rings to isolate colonies of antibiotic-resistant cells; during the time required to grease, place... below) is simple: After obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual...cells did not seem to form discrete colonies but constantly migrated away from each other. In our experience...

  13. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...immersed in an industry environment, have a better understanding of the fast, deadline-driven pace of biotech...number of steps, decisions and people involved is staggering. The process is complicated. If you’re interested... decide what they’d like to work on later. For instance, AstraZeneca posts most of its positions all at...

  14. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...each step. Factors such as enzyme efficiency, stability, and buffer compatibility have placed practical...et al. at NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over...for all 3- and 4-base overhang sequences under standard reaction conditions. This data set allows quantitation...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...glycosylation and acetylation to create functional, stable proteins. Protein expression refers to the second...can express proteins both transiently or through stable cell lines. Both methods produce high protein yields...adapted to fermentation allowing for large-scale, stable production of proteins. In general yeast expression...

  16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify and sequence the locus...into the FBL locus. Upon sequencing of puromycin resistant clones, they found that 80% and 50% of clones ...using the nuclease Cpf1. Since Cpf1 cuts in a staggered pattern, it is thought to be ideal for HDR-independent...

  17. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...could be combined effectively (3). In Figure 1 for instance, the DBD of the fusion protein shown is still ...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...subset based on a cellular compartment or growth stage). A prey library can either be applied to the bait...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...before investing time and resources into making a stable cell line. When expressing a viral construct transiently... its broad host range, and increased particle stability; however, the protein is cytotoxic precluding ...specific experiments being performed and you should understand the guidelines for using such reagents as outlined...

  19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...protein control. We also added the toxin-antitoxin stability cassette Axe-Txe so that the plasmid could be ...cells gave drastic improvement in expression. Stabilizing the gvpA transcript with WPRE reduced the number...days. GVs should be visible under phase contrast starting day 2 after transfection.     Fig: 2: Expressing...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...alternative versus plasmid-based expression which can be unstable and lack precise control over copy number. MUCICAT... 1-11 copies of integrated genes. We obtained a stable and high-yielding strain which had 6 times the ...faster construction or reprogramming of cells to understand the genetic and biochemical mechanisms behind...
Showing: 761 - 780 of 1046 results