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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,053 results for: sta

Showing: 761 - 780 of 1053 results

  1. The time and cost required to make a plasmid

    Type
    Blog Post
    ...much time you have to spend cloning before you can start your experiment? What about all the reagents you...you did everything correctly the first time – no mistakes, no repeats! How long does it take in total? While...occurred. Here at Addgene, the consumables cost per stab has increased 50% since 2019, while overall inflation...

  2. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas ...Best for: very large deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s...primary difference from Cas9 is that it generates staggered breaks, also known as sticky ends. Cas12a cleavage...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... miniature CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (...also created a miniature yet active Cas system starting with the same Un1Cas12f1 (Cas14). They noted that...and introduce epitope tags and RNA transcription start sites. The lab further demonstrated that PRIME-del...

  4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    .... coli and yeast. Because this approach uses standardized parts, it allows for greater reproducibility...CO2 into water and electrical energy generation. Alistair McCormick’s lab recently developed the CyanoGate...muscle and epithelial cells, as well as in different stages of development, ensuring the breadth and versatility...

  5. dTAG - You're it!

    Type
    Blog Post
    ...protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological...degradation in a cell line of interest prior to starting knock-in experiments. Test dTAG molecule toxicity...Small molecule degraders may recruit CRBN to nonstandard or “neo-substrates.” We advise evaluation of ...

  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... control the abundance of proteins in cells. Homeostatic regulation of protein abundance is essential ...of protein-protein interactions in situ. In the standard PLA assay, primary antibodies directed towards...microscope she likes to travel, discover new restaurants, go to concerts and bake. you can contact her...

  7. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ... our lab used jargon that required context to understand, I came to realize that there were a lot of words...a list of frequently used words like this when starting rotations or my postdoc would have simplified ...Feeling like people aren’t listening to you or understanding you can be incredibly frustrating. Clarifying...

  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... pathway of interest, but there can be a lot of static since other signaling networks can also be non-....” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure 1: Photoswitchable...turn off psRaf1). After this, the phosphorylation status of MEK1/2 was tracked at 5 minute intervals via...

  9. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types...of a single heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed...GFP regulation to other types of proteins. They started with another biological workhorse, Cre recombinase...

  10. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...for a successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who...in this group have scientific backgrounds. For a stated purpose of transitioning a scientist from academia... to Life. She runs great tweet chats, has an outstanding blog and, while her PhD is in History, I think...

  11. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral vectors are derived...Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ...lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...

  13. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... the F1 generation, your breeding scheme should start with parental mice that are each homozygous for ...chromosomes in the parental generation, they are likely to stay on separate chromosomes in future generations as...homozygous for both mutations. The shorter the distance between the two loci of interest on the chromosome...

  14. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...elegans. To study the role of 3’UTRs, the Arizona State University (ASU) Biodesign institute developed a...opportunities to develop computational methods to better understand animal development (Kyoda et al., 2012).  C. ...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    ...cell cycle attempt to understand development, regeneration, and certain disease states. However, it’s difficult... each tool expanding our ability to study and understand the complex interworking of the 86 billion neurons...

  16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ..., even if he or she is a difficult person: 1) Understand your supervisor’s expectations Every supervisor... details of your work. Describe the problem by stating the facts and explain how it is interfering with...is miscommunication. For example, you might misunderstand your supervisor’s suggestions and take your ...

  17. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...similar regions. This combination of chewing up one stand and cutting the template strand causes large deletions... to studying Cas3 and its Cascade partners to understand it and use it to its full potential. Hopefully...Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R....

  18. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degradation of the tagged protein. Degrons are destabilizing domains which will be identified by various ...protein must be expressed (inducibly, ectopically, stably… it’s your call). The TIR1 F-box protein will associate.... If your project also involves imaging at some stage, then choosing a HaloTag could pay dividends down...

  19. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic...definitive, but visual identification is a good place to start before taking on more time consuming and expensive...use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used codon optimization programs...

  20. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...each step. Factors such as enzyme efficiency, stability, and buffer compatibility have placed practical...et al. at NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over...for all 3- and 4-base overhang sequences under standard reaction conditions. This data set allows quantitation...
Showing: 761 - 780 of 1053 results