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.... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

...and Maria have lots of ideas and are happy to speculate, they know they can’t answer these questions alone...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

...     The requirement for Cas9 transcription and translation delays editing What are Cas9-gRNA ribonucleoproteins... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

...the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and meticulously...rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID...

...communities and into species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...PMC2802578. 4. Xue, Lin, et al. "Imaging and manipulating proteins in live cells through covalent labeling...

...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not...applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids...

...and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very...Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR to Quickly...

...therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus...investigate transcription factor candidates and the regulation of genes in the biosynthetic pathway. Why plant...

... of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins...proteins. Instead, consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it...

...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs...

...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

...integration), which is especially valuable for regulatory approval and public acceptance. Recently, viral...complement guide (see Figure 1). Perform standard phosphorylation and annealing of the two oligos. Figure...

...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...

...; the importance of which will be elaborated on later. As for what job fairs to consider, some of the ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...

...different opinions, and some of them would acknowledge later that without my “neutral” presence to alleviate ... that nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I...

...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... Originally reported as a monomer16, IFP2.0 was later found to be a dimer3,15. ND-not determined in parallel...

...applications, they have become indispensable tools for manipulating cells and organisms. They can be used for a ...This is because most titering methods rely on isolating genomic DNA from transduced cells and quantifying...

... require sequencing of many samples at once for later comparison. These next-generation sequencing techniques...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...

...super-resolution microscopy techniques, such as Stimulated Emission Depletion (STED), and Stochastic Optical... expand the self-labeling capabilities of this platform. The Janelia Fluor ligands provide brighter and...