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Showing: 761 - 780 of 945 results

  1. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not...applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids...

  2. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus...investigate transcription factor candidates and the regulation of genes in the biosynthetic pathway. Why plant...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs...

  4. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...; the importance of which will be elaborated on later. As for what job fairs to consider, some of the ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...

  5. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

  6. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins...proteins. Instead, consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very...Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR to Quickly...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... Originally reported as a monomer16, IFP2.0 was later found to be a dimer3,15. ND-not determined in parallel...

  9. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...different opinions, and some of them would acknowledge later that without my “neutral” presence to alleviate ... that nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    ... require sequencing of many samples at once for later comparison. These next-generation sequencing techniques...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...

  12. New Antibody Animation!

    Type
    Blog Post
    ...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

  13. New Coomassie Protocol Video

    Type
    Blog Post
    ...determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant...

  14. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

  16. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared on our ...

  18. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...the month of December are eligible to win a hot chocolate party that will warm up the entire lab. We’ll ...

  19. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...to me: enhanced prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ...week, we launched our new AAV Data Hub, an open platform for scientists using AAV to find and contribute...
Showing: 761 - 780 of 945 results