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  1. PhD Applications After COVID

    Type
    Blog Post
    ...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...

  2. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...recombination produces a minor pre-recombined population that does not require the recombinase for transgene... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...’s best to turn to an established social media platform. During the conference If the conference has a...looking for a job - those connections can help you later on. Update your LinkedIn and your resume and prepare...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...     The requirement for Cas9 transcription and translation delays editing What are Cas9-gRNA ribonucleoproteins... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and meticulously...rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not...applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids...

  9. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs...

  11. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus...investigate transcription factor candidates and the regulation of genes in the biosynthetic pathway. Why plant...

  12. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins...proteins. Instead, consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it...

  13. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...; the importance of which will be elaborated on later. As for what job fairs to consider, some of the ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...

  14. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very...Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR to Quickly...

  15. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...different opinions, and some of them would acknowledge later that without my “neutral” presence to alleviate ... that nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I...

  16. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... Originally reported as a monomer16, IFP2.0 was later found to be a dimer3,15. ND-not determined in parallel...

  17. Evolution of Lab Techniques

    Type
    Blog Post
    ... require sequencing of many samples at once for later comparison. These next-generation sequencing techniques...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...

  19. New Antibody Animation!

    Type
    Blog Post
    ...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

  20. New Coomassie Protocol Video

    Type
    Blog Post
    ...determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant...
Showing: 761 - 780 of 955 results