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...help me get the engagement I wanted. I therefore started planning a game that would force my students to...and strategy skills to apply those concepts and understand how therapeutic strategies work. Find Addgene...Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one “Transfer...

...three times, to opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited.... Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...

...begin and end with the same steps as screens – they start with manipulations (CRISPR, etc.), and finish with...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...answer your experimental question. Now that you understand the difference between the two, it’s time to ...

...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered... in 1985, luciferase assays have become a gold standard in gene expression analysis to investigate the...

...simple goal… and we had no idea how much our understanding of what accessibility is would change over the...information? This can be physical or digital. For instance, you would present information differently in ...paper accessible to even more people.  Whether you start small with alt text or immediately dive into applying...

... turn it down, knock it out, etc. It’s how we understand what genes do! Viruses have several options to...of each variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal...services and even a Viral Vectors eBook to get you started. Ready to plan your next (or maybe first!) viral...

...nanobody to its target stabilizes the TurboID-nanobody protein and allows TurboID to start labeling proteins...biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of...

...promoter using the AAV-BR1 capsid? A: It is our understanding that AAV-BR1 has the same genome size restrictions.... We are also adopting ddPCR, which does not a standard curve and somewhat more reliable quantitation....outlier for PHP.eB (1x1011 - 1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top...

...If you’re just getting started using antibodies in your experiments, you may be curious about all of ...antibodies depends on a variety of factors. Understanding the advantages and disadvantages of monoclonal...AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS...

...specific skills you need for your current position Understand your interpersonal challenges if they know some...formal mentoring program can be a great way to get started with mentoring. Read this Catalyst Report “Making... sound like a big responsibility or commitment. Start by asking them to have coffee. This is much less...

.... coli and yeast. Because this approach uses standardized parts, it allows for greater reproducibility...CO2 into water and electrical energy generation. Alistair McCormick’s lab recently developed the CyanoGate...muscle and epithelial cells, as well as in different stages of development, ensuring the breadth and versatility...

... pathway of interest, but there can be a lot of static since other signaling networks can also be non-....” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure 1: Photoswitchable...turn off psRaf1). After this, the phosphorylation status of MEK1/2 was tracked at 5 minute intervals via...

... control the abundance of proteins in cells. Homeostatic regulation of protein abundance is essential ...of protein-protein interactions in situ. In the standard PLA assay, primary antibodies directed towards...microscope she likes to travel, discover new restaurants, go to concerts and bake. you can contact her...

... our lab used jargon that required context to understand, I came to realize that there were a lot of words...a list of frequently used words like this when starting rotations or my postdoc would have simplified ...Feeling like people aren’t listening to you or understanding you can be incredibly frustrating. Clarifying...

...familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas ...Best for: very large deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s...primary difference from Cas9 is that it generates staggered breaks, also known as sticky ends. Cas12a cleavage...

...much time you have to spend cloning before you can start your experiment? What about all the reagents you...you did everything correctly the first time – no mistakes, no repeats! How long does it take in total? While...occurred. Here at Addgene, the consumables cost per stab has increased 50% since 2019, while overall inflation...

... miniature CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (...also created a miniature yet active Cas system starting with the same Un1Cas12f1 (Cas14). They noted that...and introduce epitope tags and RNA transcription start sites. The lab further demonstrated that PRIME-del...

...protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological...degradation in a cell line of interest prior to starting knock-in experiments. Test dTAG molecule toxicity...Small molecule degraders may recruit CRBN to nonstandard or “neo-substrates.” We advise evaluation of ...

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types...of a single heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed...GFP regulation to other types of proteins. They started with another biological workhorse, Cre recombinase...

...for a successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who...in this group have scientific backgrounds. For a stated purpose of transitioning a scientist from academia... to Life. She runs great tweet chats, has an outstanding blog and, while her PhD is in History, I think...