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Showing: 741 - 760 of 901 results

  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/...

  2. Viral Production at Addgene

    Type
    Blog Post
    ...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization...

  5. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field with...

  9. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st, according...centrally administered, which leads to many complications, including the inability of some institutions...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity...

  13. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live...which yields a much weaker signal on live cells compared to dead cells. As a result, the largely negative...

  14. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...

  18. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...
Showing: 741 - 760 of 901 results