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  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...

  4. dTAG - You're it!

    Type
    Blog Post
    ...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

  5. Antibodies 101: Affinity Reagents

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    Blog Post
    ...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  6. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...the requirements of the specific assay. For the latter three, secondary antibodies are indicated when ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...

  7. CRISPR in the Clinic

    Type
    Blog Post
    ...correcting monogenetic diseases. Now, almost 10 years later, CRISPR’s potential for treatment is no longer an...CRISPR to disease correction in adult stem cells isolated from two patients with cystic fibrosis (Schwank...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

  11. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...the membrane for storage, but plan to reprobe it later, strip it thoroughly first. Then continue on with...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

  12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...techniques may hold the key. In the simplest form, translating gene therapies from conceptual design to clinical... thought to be as high as 70% among the human population. These pre-existing antibodies can interfere ...

  13. PhD Applications After COVID

    Type
    Blog Post
    ...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...

  14. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...’s best to turn to an established social media platform. During the conference If the conference has a...looking for a job - those connections can help you later on. Update your LinkedIn and your resume and prepare...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...and Maria have lots of ideas and are happy to speculate, they know they can’t answer these questions alone...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  16. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...recombination produces a minor pre-recombined population that does not require the recombinase for transgene... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

  17. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not...applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids...

  18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...     The requirement for Cas9 transcription and translation delays editing What are Cas9-gRNA ribonucleoproteins... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and meticulously...rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID...
Showing: 741 - 760 of 945 results