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Showing: 741 - 760 of 955 results

  1. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...offered by the system that allows for specific manipulation of neurons. We have expanded our AAV collection...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and...

  3. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...adding more terms.  Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

  6. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...

  7. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...? Sometimes, the funders of your research may stipulate that your article must be published in an open...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...

  8. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...the requirements of the specific assay. For the latter three, secondary antibodies are indicated when ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...

  9. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  11. dTAG - You're it!

    Type
    Blog Post
    ...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

  14. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...the membrane for storage, but plan to reprobe it later, strip it thoroughly first. Then continue on with...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  17. CRISPR in the Clinic

    Type
    Blog Post
    ...correcting monogenetic diseases. Now, almost 10 years later, CRISPR’s potential for treatment is no longer an...CRISPR to disease correction in adult stem cells isolated from two patients with cystic fibrosis (Schwank...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...techniques may hold the key. In the simplest form, translating gene therapies from conceptual design to clinical... thought to be as high as 70% among the human population. These pre-existing antibodies can interfere ...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    .... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

  20. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...and Maria have lots of ideas and are happy to speculate, they know they can’t answer these questions alone...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...
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