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We narrowed to 1,024 results for: cat

Showing: 741 - 760 of 1024 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

  2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ... potential control, and provide evidence that truncated gRNAs have improved specificity with only a small...toxicity in high-throughput screens." Nature communications 8 (2017): 15178. Pubmed PMID: 28474669. Pubmed...Improving CRISPR-Cas nuclease specificity using truncated guide RNAs." Nature biotechnology 32.3 (2014):...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...controlled by collections of neurons that are intricately wired into circuits through synaptic connections...photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases background...more HA tags. 4. High-resolutions microscopy As indicated above, smFPs can be used with conventional microscopes...

  4. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...detrimental as too little, resulting in MECP2 duplication syndrome or Rett Syndrome, respectively. One ...Efficient and Precise CRISPR/Cas9-Mediated MECP2 Modifications in Human-Induced Pluripotent Stem Cells. Front..., Hector RD, Cobb SR, Bird A (2017) Radically truncated MeCP2 rescues Rett syndrome-like neurological ...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...folding and stability, change post-translational modification sites and even affect protein function (Mauro... AP, Bilen B, Zavolan M (2013) Analysis of CDS-located miRNA target sites suggests that they can effectively...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate... and plasmids. To encourage collaboration and education in protein science, IPI hosts events and training...subtype-specific β1 integrin mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038/s41467...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...setting. CRISPR is often used for animal germline modification, to repair or add in disease-causing mutations...that AAV-CRISPR does not cause obvious germline modification, an important finding given the controversy ...Blog Learn How CRISPR Could Be Used to Treat Cataracts and Cystic Fibrosis Read How Homology Directed...

  8. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...tests  Statistical tests fall into two general categories: parametric and non-parametric. In this blog ... exist somewhere within your data; it does not indicate which groups the differences exist between. For...that matter. However, this is oftentimes an oversimplification. If your independent variables are affecting...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immune response to the development of tumors is a complicated and dynamic phenomenon that is interestingly ...effects, damage healthy cells, and often cannot eradicate metastasis or eliminate the probability of a relapse...based on their level of progression, anatomical location, characteristic (solid localized tumors or systemic...

  10. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  11. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...antibody collection, as well as access to IPI educational resources developed to support use of their antibody... Addgene also offers a growing collection of educational resources on our website and blog, including ...

  12. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...these tools over the years, the amount of CRISPR educational resources hosted on our website and blog have...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

  13. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....et. al. (2022) A plasmid-based system for locating and containing escaped flies. Nature 4:1-22. https:/...

  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...With barcode tracking, you will always be able to locate materials quickly and easily. Learn more about ...Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos Page 5 Science Rap...

  15. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...easy tech transfer process to our website and educational resources. We've got twenty years of experience... you know you can search for plasmids from a publication by pasting the paper's title into the Addgene...

  16. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    .... I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the foundations...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

  18. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Born to a family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best... 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to go into...
Showing: 741 - 760 of 1024 results