...presence of the poly(A) tail targets the RNA to the degradosome, which contains enzymes that cut RNA not protected...

...CRISPRa is well-suited, including studying mRNA dosing effects, cellular reprogramming, and disease modeling...

...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...

..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...

...to remove salt, then suspend a freshly harvested dose of cells (preferably in log phase) in blocking solution...

...Choudhary A (2019) A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew...

...purified antigen to demonstrate the expected antibody dose response....probe with an antibody against the target to show a dose response. Sharing speeds science. We believe that...standard curve . If you don’t see any color change or dose response, your antibody concentration may have been... the reaction is saturated and you don't see any dose response, your antibody concentration may have been...

...titrating your antibody to determine the optimal dose. Secondary antibodies must match the host species...titrating your antibody to determine the optimal dose. To ensure that your antibody is both functioning...

...purified antigen to demonstrate the expected antibody dose response....