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We narrowed to 969 results for: Cre

Showing: 741 - 760 of 969 results

  1. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...

  2. To Each HIS Own

    Type
    Blog Post
    ...function, however longer histidine strings can increase that risk.    Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended...

  3. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

  11. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

  15. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...of antibiotics are often essential genes which creates a challenge when studying them since their complete...

  19. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...your team you will have a much better chance of creating a high-performing team - which is every manager...! Remember that delegating is not just a way to create more time for yourself, but it will help develop...

  20. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... you have either made a pipetting error or an incredible discovery. The range of RQ values used to call...to be amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination...
Showing: 741 - 760 of 969 results