Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 969 results for: Cre

Showing: 741 - 760 of 969 results

  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less...

  3. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

  9. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

  11. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

  17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...of antibiotics are often essential genes which creates a challenge when studying them since their complete...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Pre-made viruses have become increasingly accessible and are useful for saving time and avoiding potentially... collected over the course of a couple days to increase yield. The collection process is a cycle of collecting...

  20. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... you have either made a pipetting error or an incredible discovery. The range of RQ values used to call...to be amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination...
Showing: 741 - 760 of 969 results