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  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...variant has similar photostability and brightness compared to the original, though its maturation is even...cell-specific tropism, with slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized...

  2. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...required to produce your fiber optic implant. Companies such as Thor Labs and Precision Fiber Products...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    ...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments that are not fluorescent on...

  7. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    .... The donor and acceptor fluorophores must be compatible, such that the emission spectrum of the donor...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...

  10. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...

  12. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  13. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...protein fusion. CRISPR genome editing can help accomplish this goal. The power of this system lies in the...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...gauged interest from relevant parties, and the company at large, by polling and informal communications...

  15. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

  16. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

  17. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology...time. Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  20. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...using advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind...
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