Addgene

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Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

...proteins, finding that APOBEC3A and APOBEC3bctd are the most efficient (Martin et al., 2019). TLDR: The eGFP ...
CRISPR Activation: A Practical Guide

Type

Blog Post

...the scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there...
Data Freedom: The Expansion of Data Sharing in Research Publications

Type

Blog Post

...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid in bacteria (Ahmed...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...the orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where ...
CRISPR 101: Validating Your Genome Edit

Type

Blog Post

... need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion large enough...
Tips for Getting a Faculty Position

Type

Blog Post

...research. Still a Grad Student? Get Tips on Getting the Most out of the Process Get more tips from the full eBook...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

...can be difficult to compare their performance, as most translation initiation sequences are optimised for...
Site Directed Mutagenesis by PCR

Type

Blog Post

...Consequently, site directed mutagenesis is one of the most popular approaches for introducing minor modifications...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control...
Technologies Enabled by NanoLuc® Luciferase

Type

Blog Post

...fluorescent protein. FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers...
Plasmids 101: Restriction Cloning

Type

Blog Post

...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...
Antibodies 101: Flow Cytometry

Type

Blog Post

...microscopy must be used. To learn more about the most suitable fluorescent microscopy technique for your...
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Type

Blog Post

...expressing Cre. The result of a system like this is a mosaic of cells labelled with different fluorescent proteins...
CRISPR 101: Cytosine and Adenine Base Editors

Type

Blog Post

...four adenine base editors (ABEs). ABE7.10 is the most active editor, displaying an average editing efficiency...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

...Rather than reprogram cells all the way back to their most primitive pluripotent stem cell state, through transdifferentiation...
Optogenetics + CRISPR, Using Light to Control Genome Editing

Type

Blog Post

...photoactive Cas9 tool, as shown in the figure below. Their most successful design utilized Magnet photoswitchable...
Optogenetics Guide

Type

Guide

...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...

Showing: 721 - 740 of 740 results

Results per page:

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

CRISPR Activation: A Practical Guide

Data Freedom: The Expansion of Data Sharing in Research Publications

Antibodies 101: Single Chain Fragment Variables (scFvs)

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Deep Mutational Scanning with One Pot Saturation Mutagenesis

CRISPR 101: Validating Your Genome Edit

Tips for Getting a Faculty Position

Multiple Plasmids at a Low Price: Kits from Addgene

Site Directed Mutagenesis by PCR

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Technologies Enabled by NanoLuc® Luciferase

CRISPR 101: Epigenetics and Editing the Epigenome

Plasmids 101: Restriction Cloning

Antibodies 101: Flow Cytometry

Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

CRISPR 101: Cytosine and Adenine Base Editors

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Optogenetics + CRISPR, Using Light to Control Genome Editing

Optogenetics Guide

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