Addgene

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Tips from the Repository Trenches: Using Barcodes to Track Samples

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Blog Post

... different cells than were originally requested. Most of our samples (98.2%) transform the first time ...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...career path. A part of the career path story for most established grant professionals is learning to succeed...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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Blog Post

...Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...directed repair, since HDR-mediated gene editing is most efficient when target sites are located in close...
Finding nucleic acids with SHERLOCK and DETECTR

Type

Blog Post

...type of readout allows for nucleic acid detection almost anywhere as lateral flow strips are easy to transport...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...same replication control machinery within the cell. Most plasmids encode a negative regulation system involving...
An Inside Look into Addgene's Dev Team

Type

Blog Post

... third party libraries and any security updates. Most of the time, updating these libraries is straight...
Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

...studies have been essential for this task. However, most lesioning methods destroy areas larger than intended...
Save Time with Transient Plant Leaf Transformations

Type

Blog Post

...tissue (see Figure 3). This method is probably the most scalable method. Transfer your plant into the dark...
Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

...multiplex sample, the cut site will be aligned to the most 5’ cut site. Indel Distribution In the Indel...
Transferable Skills Guide: External Collaborations

Type

Blog Post

...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship...
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Type

Blog Post

...high concentration of FLEx vectors guarantees that most cells are transfected with all labeling plasmids...
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

...first try to produce GVs in their own lab using the most reliable method, which is the transient cotransfection...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...compatible PAM at your site of interest, that is the most efficient option. If there is no NGG in your genomic...
Hot Plasmids Spring 2024

Type

Blog Post

...SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins and a critical component for viral...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

... plasmids all replicate in E. coli, which is the most commonly used bacteria in lab manipulations. Table...
Hot Plasmids - November 2023

Type

Blog Post

...undesired edits (indels, etc.), while PE6c and PE6d were most efficient at complex edits, such as long or highly...
Viral Production at Addgene

Type

Blog Post

...This article was written by multiple Addgenies and most recently updated by Ina Ersing in August 2023. ...
Antibodies 101: The Four ELISAs and When to Use Them

Type

Blog Post

...source of cross-reactivity. Sandwich ELISAs In the most common type of ELISA, the sandwich ELISA, users ...
Transferable Skills Guide: Creativity

Type

Blog Post

... tendency of a group of people to agree with the most prominent idea, and instead building an environment...

Showing: 701 - 720 of 740 results

Results per page:

Tips from the Repository Trenches: Using Barcodes to Track Samples

A Career in Grants: How To Become a Grant Professional

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Finding nucleic acids with SHERLOCK and DETECTR

Plasmids 101: Plasmid Incompatibility

An Inside Look into Addgene's Dev Team

Deciphering the Mysteries of Behavior with Viral Vectors

Save Time with Transient Plant Leaf Transformations

Analyzing CRISPR Editing Results with ICE from Synthego

Transferable Skills Guide: External Collaborations

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Hot Plasmids Spring 2024

Plasmids 101: Broad Host Range Plasmids

Hot Plasmids - November 2023

Viral Production at Addgene

Antibodies 101: The Four ELISAs and When to Use Them

Transferable Skills Guide: Creativity

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