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Showing: 701 - 720 of 970 results
  1. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...function, and C. elegans overexpression plasmids to upregulate their expression in specific cells. With a more...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...
  2. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...gives a presentation on what they do and how it relates to other teams at the company. This provides new... I know some fear that remote work would feel isolating, but that has not been the case for me at Addgene...
  3. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...
  4. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...
  5. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...
  6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...
  7. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...
  8. Fostering communication in remote collaborations

    Type
    Blog Post
    ...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...
  9. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...
  10. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ...
  11. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...
  12. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...
Showing: 701 - 720 of 970 results