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TypeBlog Post...function, and C. elegans overexpression plasmids to upregulate their expression in specific cells. With a more...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...
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Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists ...resources on the Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19... -
Working 9-5: an Addgene Co-op
TypeBlog Post...gives a presentation on what they do and how it relates to other teams at the company. This provides new... I know some fear that remote work would feel isolating, but that has not been the case for me at Addgene... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...citing plasmids from Addgene help others find them later if they need to use them in their own work, it also...should make it easier for them to find your work later. We also hope that this feature will decrease redundancy... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously established... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...is pictured here…she started from a blank white slate and developed this over 30 minutes, which was fun...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...game for anyone looking to quickly and easily manipulate specific genes. But what if you want to study...said they were able to produce a very diverse population of cells within a lab dish in a matter of a few... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...identified proteins and enzymes. These compounds will later get tested for their potential agricultural and ...agriculture. For this project the expression of isolated bacterial proteins can be achieved using a bacterial... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we ...size of the plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the... -
Fostering communication in remote collaborations
TypeBlog Post...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ... -
Antibodies 101: Affinity Tags
TypeBlog Post...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post... data, and I was given a fantastic spreadsheet template to build out a report on. I was able to understand...cultivates trust between coworkers and that trust then translated into this incredible experience. Of course, ... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...