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  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...

  2. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...LitSuggest Tool Following the “rabbit hole” of related cell lines when annotating a cell line Community...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever...

  3. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

  4. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...

  5. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

  6. Fostering communication in remote collaborations

    Type
    Blog Post
    ...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

  8. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

  13. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  14. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...

  16. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

  18. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...companies.  The majority of these meetings acted as a platform for significant company advancement. Be relentless... you’re able to answer every possible question related to it. First impressions are everything and you...

  20. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...process in detail often suggests new strategies to manipulate that process. We began our research with a simple...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...
Showing: 701 - 720 of 945 results