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  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids...living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in this early example...

  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...

  5. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

  9. Fostering communication in remote collaborations

    Type
    Blog Post
    ...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

  11. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ...

  12. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...

  13. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...LitSuggest Tool Following the “rabbit hole” of related cell lines when annotating a cell line Community...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever...

  16. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...endothelial cells via an injection into the systemic circulation. In Old World monkeys it actually targets neurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

  17. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...

  18. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  19. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

  20. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...
Showing: 701 - 720 of 955 results