...thicker as the proteins and DNA are denatured. Pro-Tip Do not vortex at this stage or the genomic DNA will ...4. Note: Phenol-chloroform is a hazardous waste - DO NOT pour down sink. Top Protocol: Ethanol Precipitation...

...Media 10 mL 10% Pluronic-F68 40 mL 200 mM Glutagro Do not add selective reagents Store at 4 °C until use...incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that are over 30 passages. Section ...

...complementary to template strand). However, primers do not need to correspond to the template strand completely...

...scrape some of the frozen bacteria off of the top. Do not let the glycerol stock unthaw! Streak the bacteria...

...surface. Tare the weighing boat or paper. You need to do this because you don’t want to include the weight...

... a new or experienced reprogrammer? Which methods do you have experience using? Let us know about your...multiple transfection makes it difficult to control the dose of plasmid the cells receive over the whole reprogramming...

...entire dilution plate with Microseal adhesive seal - do not press the film, just gently cover so nothing ...collecting drops along the side of the tube. Cap gently - do not push the cap in all the way, just ensure the ...

...the surface). We use the micropipette tip itself to do the spreading; the tip is gently bent to create an...

...of the tube. This way, as items are floating, you do not need to necessarily maneuver the bottles or tubes...

... significant medical interest, however efforts to do so have been hindered by a lack of tools, compounds...target soluble kinase domains, but these domains often do not express well in E. coli without their partner...cells. The mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells...and not specific loci. To overcome this flaw, the Zoldos lab has developed CRISPR tools for epigenome editing...and their effects on gene regulation. Professor Zoldos and colleagues from the Department of the Biology...deletion efficiency was dependent upon 1) Cas9 and gRNA dose (both of which can be modulated using the different...

...designed by Eric Greenwald and the Jin Zhang Lab at UCSD Do you have suggestions for other plasmids that should...Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic GMP) Green fluorescent probe for ...

...the vector with EcoRI and SalI (see diagram ). To do this, we add 5' - AATTC and G - 3' to the top oligo...

...Protocol Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...

... centrifuge and be dangerous for the user. If you do not have a balanced number of samples, you may need...

...Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise the ion pump’s function or cause cellular...

...images directly to a computer, but many microscopes do not and you may have to dust off your drawing skills...

... and a negative control sample such as cells that do not express the protein of interest....titrating your antibody to determine the optimal dose. To ensure that your antibody is both functioning...

...8x10 5 cells in a T75 flask in a volume of 15 mL. Do not add antibiotics to the media. Use cells that ...

...10 5 cells in a T75 flask in 15 mL DMEM Complete. Do not add pen-strep to the media. Use cells that are...

...class II, DO alpha HLA-DNA, HLA-DZA, HLADZ HLA-DOB major histocompatibility complex, class II, DO beta DOB...180015. doi: 10.1038/sdata.2018.15. PMID 29485622 Do you have suggestions for other plasmids that should...