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  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...CellTags for tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating...

  3. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...

  4. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...epitopes available if it is being selected out of a complex mix of proteins that have been extracted from a...Picking the Right Antibody  It can get even more complex if you’re looking for proteins that are interacting...

  5. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...

  7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ...secondary structure and high GC content, but some companies now offer Sanger sequencing conditions which can...

  8. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...individual diversity to get from gamete to a visibly complex - and unique - adult. My initial research directions...evolutionary perspective brought by studies of comparative anatomy showed me that some of the most fascinating...

  9. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Musicophilia is accessible and fascinating, all without compromising on the relevant science."     - Randall Carter...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...

  11. Hot Plasmids - January 2023

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    Blog Post
    ...   Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head for gene expression changes of non-target...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...

  12. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius...

  14. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...playing a card game called “Magic”. There were complicated rules that governed how this game was played....should be “brains-on”, but as much as I try being compelling, interactive, and application-driven to boost...

  15. The MTA at Addgene

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    Blog Post
    ...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

  16. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in... are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 ...

  17. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

  19. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...custom primers or "primer-walking" reactions are completely unnecessary for sequencing multi-kb DNA inserts...seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

  20. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...
Showing: 681 - 700 of 901 results