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  3. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...These tips were written early on in the pandemic. Later in the year, Joanne Kamens followed up with a blog...mapping, DNA/protein databases, DNA sequence manipulation, and primer design. Best wishes for the new...

  10. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

  14. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...gives a presentation on what they do and how it relates to other teams at the company. This provides new... I know some fear that remote work would feel isolating, but that has not been the case for me at Addgene...

  15. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...function, and C. elegans overexpression plasmids to upregulate their expression in specific cells. With a more...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...

  16. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...

  17. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids...living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in this early example...

  18. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...

  20. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...
Showing: 681 - 700 of 945 results