We narrowed to 968 results for: Cre
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TypeBlog Post...happen anywhere!). The pair quickly teamed up to create Phage Directory in a whirlwind three days in November...purified before sending the phages to the clinicians. Creating a repository of phage data and information Since...
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Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...for research by anyone. As infection rates were increasing in the United States, and many universities and... community speed research is to offer plasmids created as part of research projects, another way is to... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post... are we! This expansion will give researchers increased flexibility when planning their research and expanded...This split allows us to provide researchers with increased choice and flexibility; while ensuring we can ... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post... to GRABNE, and having both colors allows for increased options for multiplexing in experiments, including...both nLightG and nLightR showing significantly increased selectivity for norepinephrine over dopamine. ... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...the possible science careers. She advocated for increasing the value and priority put on good mentoring ... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...proposition, explained Michelle Baird, who has created plasmids as a technician in the Davidson lab for...prompted him to deposit the tools he and his team created with the research community at large, "[Fluorescent... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...accurately reflect our culture and outlook: Addgene increases productivity by providing world-wide access to...leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters a culture... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA, for example...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...operons from two different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...DNA-protein interactions, the reliance on fixation does increase the chance of false-positives, as proteins may...RUN doesn’t require fixation and significantly decreases background noise, reducing the read depth necessary... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post... to help you do better research. CRISPR is an incredibly fast-moving field, and we want to make it easy...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality... -
Sensing Neuronal Dopamine
TypeBlog Post...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: ... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula...alternative science careers here Interested in creative science education tools? Check out this Addgene... -
Year of the Camelid: Antibody Style
TypeBlog Post...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation. ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021.... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...critical thinking and problem solving abilities, increased my technical know-how, and helped develop my science...scientific knowledge and technical know-how that increased my autonomy and satisfaction with my work. 5.... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...leader’s job to provide the driving inspiration, to create a productive culture and to correct systemic problems...try operating in a different role? I think the secret sauce can be summed up in one word – initiative... -
Choosing Your Perfect Empty Backbone
TypeBlog Post... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...your applications and experimental design. You can create a new strain with a disrupted gene by transforming... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...1989; Ozturk and Palsson, 1991; Oh, 1993) and increased yields in adenoviral (Coroadinha, 2006) and retroviral...AAV production yields had yet to be examined. Increased yields with sucrose supplementation is serotype... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...interest without an increase in the number of steps in the protocol. IgnaviCas9’s incredible thermostability... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...fluorescent signals in in vivo imaging: the 10xHA tag increased the fluorescent signal to noise ratio five fold...scaffold and HA-binding regions, the Stasevich lab created a versatile tool that can be used to visualize ...