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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 773 results for: Dos

Showing: 661 - 680 of 773 results

  1. DNA Purification Without a Kit

    Type
    Blog Post
    ... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...

  3. CRISPR Antimicrobials

    Type
    Blog Post
    ...become resistant to? CRISPR may provide a method for doing just that. While challenges remain in the delivery...

  7. Optogenetics Guide

    Type
    Guide
    ...excitation or optogenetic inhibition. First things first: do you want to turn ON or turn OFF neurons in your experiment...Examples: iChloC, SwiChRca, Phobos, Aurora Browse Channelrhodospin plasmids . Halorhodopsins Halorhodopsins are...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

  10. CRISPR Library Amplification

    Type
    Protocol
    ...total plasmid DNA. Tips and Troubleshooting What do I do if my transformation efficiency is not high enough... Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library? Introduction...Endura from two separate transformations). Pro-Tip Do not pipette repeatedly or mix when removing SOC containing...Critical Be careful not to rip or shred the agar. Do so by gentle spreading. Some spreaders have a sharp...Maxi Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately...colder reagents (not including the recovery media!). Do not proceed with Maxipreps or NGS until adequate ...representation is maintained. Maxipreps - Less is more: Do not overload the Maxipreps as yield can dramatically...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...separate non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Davidson Fluorescent Protein Collection . Return to top Do you have suggestions for other plasmids that should...14437 mRFP-Rab5 Early endosomes RAB5A mRFP Ari Helenius 61801 GFP-Rab4B Early endosomes Rab4B AcGFP Gia Voeltz...49201 mCh-Rab5 Early endosomes Rab5 mCherry Gia Voeltz 49147 BFP-Rab5 Early endosomes Rab5 BFP Gia Voeltz...GFP-Rab5B Early endosomes Rab5B AcGFP Gia Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP...pTag-BFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagBFP James Johnson 13050 DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Richard...GFP-EEA1 wt Early endosomes EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia...61804 mCh-Rab7A Late endosomes Rab7a mCherry Gia Voeltz 61803 GFP-Rab7A Late endosomes Rab7a AcGFP Gia Voeltz...

  13. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... given sequence. You want to choose enzymes that: Do not cut within your insert. Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful...GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut DNA efficiently at the end of a linear piece...any 6 bases, but you should ensure that the bases do not result in the formation of a hairpin structure...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

  14. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...want to choose enzymes that: Flank your insert, but do not cut within your insert Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will result in your...you cannot find enzymes that meet these criteria, do not fear. You have other options, such as: Adding...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

  16. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...less stable than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997)...
Showing: 661 - 680 of 773 results