Skip to main content
Addgene
Showing: 641 - 660 of 901 results
  1. CRISPR Cheat Sheet

    Type
    Blog Post
    ...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...
  2. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...decorated office! Below, they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem ...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...
  3. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009...
  4. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...Science Translational Medicine have made their complete collection of relevant papers freely available...
  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...technique and be able to talk and own these accomplishments. Employers and academic departments want to...
  6. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells. The Feng Zhang...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...
  7. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...which decrease the rate of off-target genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE... increased editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian...
  8. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ... online calendar and can be accessed from your computer or smartphone. Evernote allows you to create notes...ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to adapt, likely...
  9. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications...
  10. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity...
  11. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    .... “Initial and sustained motivation is a huge component of success.” 2. “Enjoy Teamwork.” Be friendly...friendly, open, sharing, rather than intensely competitive. Be curious and interdisciplinary.   3. “There are...
Showing: 641 - 660 of 901 results