We narrowed to 1,045 results for: sta

...observed in the kidneys at a specific developmental stage you could infer that the gene which GFP was monitoring...Development-specific genes – if a gene functions at multiple stages in development, its depletion may result in lethality... the depletion of one another. Under these circumstances, trap methods may be the preferred screening... in fact, they can be activated a considerable distance away from a gene which can make identification...

...of the manager is not to empower but to remove obstacles.” – Scott Adams, Dilbert cartoonist What makes...for example. So aside from a focus on removing obstacles, what makes a good manager? First, it takes an...consultation, advice and brainstorming. Employees must understand the part they play in the goals of the organization...their present positions By removing the primary obstacle of job dissatisfaction, the work can get done,...

...Not sure where to start? Need a quick reference? Addgene's eBooks are here to help!... Collection Not sure where to start? Need a quick reference? Addgene's eBooks are here to help! 0 of 0...

...several years ago we partnered with Seqwell to establish a next-generation sequencing (NGS) QC platform...including only titration of AAV particles and a protein stain to assess purity. For labs that produce a small ...samples, serotypes, or both. Unfortunately, the standard AAV QC regimen does not address this.  Previous...analysis approach can be easily incorporated into standard QC regimens and will provide scientists with the...

...expression of XFPs. Any longer and the cells may start to show morphological abnormalities due to the excessive...Clearing large tissues - Fluorescent proteins are very stable in SeeDB2. However, SeeDB2 is not powerful enough...neuronal circuit tracing.     Marcus Leiwe is an assistant professor at Kyushu University. He has a particular...17972876. Kohl, Johannes, et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National...

...detection assays, in vitro transduction, and silver stain approaches we show that there is no difference in...research community.    So, why are we so excited? For starters, we believe that this approach can be adapted ... of retroviral vectors: Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006)...PubMed PMID: 16528756. 2. Oh, Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic...

...insert. Since the standard ligation reaction is fairly quick, make sure you stay organized and prepare...TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction sites...Reaction into Competent Cells: You can use your standard lab protocol for this; however, you should reduce...

...we are cloning an ORF, we want to clone from the start codon (ATG) to the stop codon (TGA, in this example...give specificity and to also be compatible with a standard PCR reaction (see PCR Video). Therefore, our Forward...reverse complement to get PCR amplification. We can start similarly, taking the final 18 bases of the ORF,...restriction cloning post. However, even more than with standard restriction cloning, it is essential that you ...

...field of science you are communicating from. For instance, a plumber, a physicist, an ambassador to Spain... an email without a recipient, so why would you start a communications project without an audience?  An... racial/ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed...audience allows you to create common ground and understanding that you can use to get your message across....

...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for... to contribute to the catalog. The Catalog Will Start With Neuroscience Antibodies and Grow From There...Email [email protected] to learn more or get started.  If you have suggestions about how to make the...Bradbury A, Plückthun A (2015) Reproducibility: Standardize antibodies used in research. Nature 518:27–29...

... are focusing on tagging for detection purposes (stay tuned for our affinity purification tag blog!). ...benefits – as discussed above – but there are a few instances when they should be used with caution or not at...Protein folding issues, steric hindrances, and destabilization can be brought about by the tag so it’s important...aggregation of your protein of interest!   Ready to start epitope tagging?   References and Resources References...

...usage has been limited by technical problems. For instance, it’s much more difficult to introduce a gene ...primary (hESCs) and cultured cells showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency... adapted from D’Astolfo et al. In certain instances, the potential benefits of nonviral transduction...protein purification (once a protocol has been established) may be less laborious than viral purification...

...a great start to a mentoring relationship, but if you don’t take this past the chatting stage you will...list available from this post. Use an activity to start a discussion. The Forced Choice Analysis is a fun...clarify and discuss values. The activity is just the start – it is the discussion after that will lead to learning...

...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...choosing a protein, we often direct them there as a starting point.  However, there is a great deal more information...database relating to that specific protein (for instance: EGFP).  Users can “favorite” certain proteins...of FPbase FPbase is still very much in the early stages of development, and there is plenty of room for...

...is fine for its goal of creating a choice of standardized constructs and tools for the rational and simple...Golden Gate Assembly also allows the creation of standardized DNA constructs, but its use of Type IIS restriction...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered... in biofuel generation, green chemistry and understanding the minimal genome. Many of these techniques...

... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...antibodies, because of effects of pH on protein stability (Murphy et al., 2013). However, the pH changes...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...der Geer P, Manning MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat...

...at odd times, new strategies can make socially distanced work more creative. An example could be making...signal the approach of a brain break, where everyone stands up, disappears from the video, and prepares a coffee...brainstorm on data in smaller groups, where for instance the presence of the PI in the main room inhibits...these weird times of shift working and social distancing. The take home message is the approaches/tools...

...give rise to a starfish shape. You can ask me about the different arms of this starfish sometime, but... plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne Kamen's LinkedIn... it is completely impossible for me or anyone to stay in touch with all of their connections on a regular...

...different water filtration system, environmental stability in an incubator), but there’s a lot you can do...other researchers to help them troubleshoot and understand. All of this communication and sharing fits together...seriously. That’s why at Cell Press we developed STAR Methods to improve the structure and transparency... of checklists and guidelines to help authors understand what they are expected to do. All of that helps...

...abundance, the eGFP will be stable and fluoresce. But if the variant is unstable, it will be degraded, leading... of fluorescent reporters. Bacteria containing unstable fluorescent proteins under the control of promoters... cell cycle and growth rate using TIMER and an unstable fluorescent protein reporter under the control...