...electrical charges propagated through multiple cells to do things like translate perceived signals to physical...

... used the library to identify RBD mutations which do not interfere with ACE2 binding, but impact recognition...

...CRISPR-Cas system they block can be found here. How do anti-CRISPR proteins work? Anti-CRISPRs proteins ...

...Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ...

...ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, ...

...views in this post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene...

...Additional protein tagging systems For those who do not work with C. elegans, several groups have designed...

...it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify...

...more about xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there...

...Integrative and conjugative elements (ICEs): what they do and how they work." Annual review of genetics 49 ...

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

... given sequence. You want to choose enzymes that: Do not cut within your insert. Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful...GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut DNA efficiently at the end of a linear piece...any 6 bases, but you should ensure that the bases do not result in the formation of a hairpin structure...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

...want to choose enzymes that: Flank your insert, but do not cut within your insert Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will result in your...you cannot find enzymes that meet these criteria, do not fear. You have other options, such as: Adding...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...