...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...subsequently isolated by affinity purification against the epitope tag. Compared to conventional methods...Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow Molecular Tools ...

...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...a much lower efficiency than NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. Do you have...

...vectors, the target site sequence can be added by inserting an oligonucleotide linker into BsaI digested vector...for generating gRNAs 106.5 KB flyCRISPR website contains additional information CRISPR Optimal Target Finder...other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. (Also known as ...generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed....

...Addgene Advisory Board member, and Institute Scientist at the Broad Institute, John Doench. CRISPR technology...prime editing For a specific edit, such as the insertion of a fluorescent tag or the introduction of a ...the Genetic Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve...10.1038/s41576-018-0059-1 Veres A, Gosis BS, Ding Q, Collins R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski...

...Zhang, J., Chen, C., Papworth, G., Watkins, S., Trucco, M., Robbins, P. D., Li, J., & Xiao, X. (2006).... sometimes vary between target species or even strains. Pro tip! Getting a vector into a particular cell...growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin...tropism varies both between species and even between strains of one species, so be sure to check the literature...

...the genome. When using piggyBac™, the cargo is inserted into the genome fully intact, while, with a standard...excision-only piggyBac™ transposase (PBx), which contains mutations making it competent for excision, but...specific point mutations by gene editing. First, instead of using piggyBac™, you could use recombinase technology...been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use a single-stranded...for the isolation and sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. 5, pp. 789–...

...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically ...activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors...

...sciencedaily #instascience #ladies #research #cardiovascularresearch #parkinsonsdisease A post shared ...red nosed reindeer.       View this post on Instagram     Eppi the red nosed Reindeer! 🦌🦌 as you.... If we've tagged you it's because we love your Insta!! #LILCChristmas . . #lab #ladiesinlabcoats #Christmas...

...blog: Plasmids CRISPR Viral Vectors Fluorescent Proteins Science Careers You can use the dropdowns in ...awards that we offer there. Each topic category contains a overview page to help you get aquatinted. Here...comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. Be the first to...articles on the Addgene blog by subscribing to instant alerts below.  ...

...resources are not. Through its flagship program, Instrumental Access, Seeding Labs partners with donors to...opportunities, publishing assistance, and more to Instrumental Access participants. Like Seeding Labs, Addgene...plasmids to research labs that have received Instrumental Access equipment from Seeding Labs and that ...Global Plasmid Distribution Check out the List of Institutions that Deposit their Plasmids with Addgene ...

...between homologous pairs before viral packaging even begins. This spontaneous recombination persists even in... when the optimal time to conduct an experiment begins. This can save you time in the long run since not...accuracy and reproducibility of the data and can instill greater confidence when interpreting the outcome...https://doi.org/10.1128/jvi.02213-17 Fischer KB, Collins HK, Callaway EM (2019) Sources of off-target expression...recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. Proc Natl Acad Sci USA 116:27001...

...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit type...identified Cas14, a family of small DNA-targeting Cas proteins. Cas14 targets and cleaves ssDNA which they have...Now Dominik Niopek’s lab has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining...

...In the third installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris... Koeris did his graduate work in Professor Jim Collins' lab (then at Boston University, now at MIT) where...Professor Timothy Lu (a graduate student in the Collins' lab at the time) got the idea to engineer bacteriophage...1:25 - 6:19 : Dr. Koeris' graduate work in the Collins' lab 6:19 - 8:26 : Dr. Koeris' reasons for wanting...

...Transformation grade DNA  Transformation is the process of inserting exogenous DNA in a microorganism. The amount of...cloning includes a set of techniques that are used to insert recombinant DNA from prokaryotes or eukaryotes ...from other nucleic acids (genomic DNA and RNA), proteins and chemical contaminants that have been used ... grade DNA. Transfection is the process of inserting an external plasmid in mammalian cells and it ...

... L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations A more efficient method...regenerative medicine. iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they ...Ottosson Stem Cell Research Plasmids This table contains a general list of plasmids that have been used...name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions...

... accomplished by using, for example, red light instead of blue to excite a molecule of GFP. Because red...a host of tools and opened doors to many great insights, it can be overwhelming to a novice microscopist...from a well-established microscopist at your institution when heading into a new set of experiments. ...Lightsheet Microscopy Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent... fluorescent proteins...

...wider community of users.  We also made scientific gains by adding long-read sequencing capabilities to complement...quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al...R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B...

...Technique: Probe Phage Genomes for Host Binding Proteins & Running for Rare Disease, Running for FOP, Running...Technique: Probe Phage Genomes for Host Binding Proteins," was contributed by guest blogger Jessica Sacher...describes a technique you can use to find phage proteins that bind to your bacterium of interest. For those...an ingenious but simple technique to screen for proteins that could be useful for a wide variety of reasons...

... suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from rare diseases...Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...Elia Di Schiavi is a Research Scientist at the Institute of Biosciences and Bioresources in Naples, Italy...interactions with other genes. He will use fluorescent proteins to monitor the localization of these genes, CRISPR...