...families of cell surface and secreted proteins (glypicans and integrins) and common epitope tags, as well ...and secreted proteins from a collaboration between Addgene and IPI. Antibodies... Antibodies Institute for Protein Innovation... Innovation Antibody Collection Institute for Protein Innovation Antibody Collection Glypican Antibodies...Tag Antibodies Antibody Plasmids Welcome to the Institute for Protein Innovation (IPI) Collection! Here,...IPI to generate antibodies to highly conserved proteins and other difficult-to-target antigens that are... Recommended Applications Integrin Antibodies Integrins are a family of mechanotransduction receptors,...

...Signaling Strains Browse Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial...Strains for use in Synthetic Biology. Plasmid... Plasmid Collections Synthetic Biology Bacterial Strains Synthetic...Synthetic Biology: Bacterial Strains SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio... Bacterial Strains Search the table by keyword or sort by the table headings. Click on the publication... this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back to Top...

...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of ...utilizing one of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This...Cloning Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid...R., Abraham, J., Hensley, A., Jones, K. L., & Brzezinski, J. A. (2021). Initiation of Otx2 expression ...Goodson, N. B., Kaufman, M. A., Park, K. U., & Brzezinski, J. A. (2020). Simultaneous deletion of Prdm1...

...receptors and secreted proteins. The plasmids encode both the light and heavy chains of mouse monoclonal ... zebrafish cell surface receptors and secreted proteins. Plasmid...monoclonal antibodies to zebrafish proteins and the antibodies are produced by transfecting mammalian cell lines...

...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Depositor Collections Allen Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid...Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science has produced the first publicly... will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines...additional cell line information from the Allen Institute for Cell Science data portal (Link opens in a ...cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP...114406 SMC1A-mEGFP AICSDP-57 mEGFP SMC protein 1A Cohesins 114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin...

...probasin Mammalian Green 8405 p240 pPr-CREM/Ins CREM with insulators probasin Mammalian Green 8406 p252 pPr-CREM... p255 pPr-CREM/Ins/CMV-STOP-luc CREM and CMV-STOP-luc cassette, separated by insulator probasin Mammalian...coexpression Retroviral Littman 24333 ins-cre_cryaa-Venus Cre-Venus fusion insulin Zebrafish Stainier 24593 AAV-pgk-Cre...for inserting promoter none Mammalian Heller 112616 pmTHC TFP and Cre-ERT2 with MCS for inserting promoter...recombinases that are less toxic in Drosophila hsp70 Insect Rubin 32606 Ttr:Cre Cre expressed in visceral endoderm...CMV AAV Gage 49111 pEMS1980 iCre with MCS for inserting promoter none AAV Simpson 49455 pSH62-EBD Cre-...troponin T AAV Pu 70120 pEMS2159 iCre with MCS for inserting promoter, WPRE none AAV Simpson 72255 pCDH-CB-...

...GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's L. Miguel...Miguel Martins 14124 pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2...Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's L. Miguel Martins 15940 pcDNA3-HtrA2...Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's L. Miguel Martins 16155 pET20b-...His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-...

...inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both... edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor DNA at “...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...

...four protein chains: two large heavy chains and two small light chains. These four domains come together...their monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin ...ankyrin repeat proteins. These are antibody mimics instead of modified antibodies and are not closely related...synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead of the antibody...Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE gel to separate proteins by size...your antibody’s specificity. A blank (contains water or buffer instead of a biological sample), which captures... lab to specifically and tightly bind to other proteins. Antibodies, produced as part of the immune system...

...Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly Insertion of target... Renilla Insertion of target sequence in the 3' UTR David Bartel 12177 pIS2 Renilla Insertion of target...improved normalization. John Atkins 167576 mpCHECK2 Firefly/Renilla Insertion of 3' UTR sequences downstream...construct that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return ...Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled...organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate...elements on gene expression. Empty backbones for inserting regulatory elements before or after the luciferase...

...Plasmids 101: E. coli strains for protein expression Plasmids 101: Common Lab E. coli Strains Why Bacteria? E...Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors with N and...Expression: contains mEos2 with an N-terminal His tag. Photoactivable fluorescent proteins like this one... 3OC6HSL (Quorum sensing molecule) Cynthia Collins Contains luciferase inducible by the quorum sensing...factor bind to a particular sequence? Do these two proteins interact? What happens when I overexpress this...From recombineering to CRISPR , this collection contains a variety of tools to help you harness bacterial...binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding...

... E MGC4638 INS insulin ILPR, IRDN INS-IGF2 INS-IGF2 readthrough transcript - INSL3 insulin-like 3 (Leydig...MGC119819, RLF, RLNL INSL4 insulin-like 4 (placenta) EPIL, PLACENTIN INSL5 insulin-like 5 MGC126695, MGC126697...MGC126697, PRO182, UNQ156 INSL6 insulin-like 6 RIF1 INSR insulin receptor CD220, HHF5 JAG1 jagged 1 (Alagille...communication between cells of the immune system. Interleukins Interleukins are cytokines that are secreted by immune... omega 1 - IGF1 insulin-like growth factor 1 (somatomedin C) IGF1A, IGFI IGF1R insulin-like growth factor...MGC18216 IGF2 insulin-like growth factor 2 (somatomedin A) C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974 IGF2R...bA12D24.1.1, bA12D24.1.2 RLN3 relaxin 3 H3, RXN3, ZINS4, insl7 RNASE2 ribonuclease, RNase A family, 2 (liver...

...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...system. Within the bacterial genome, a CRISPR array contains many unique protospacer sequences that have homology... potential target sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types...

...loops at tetraloop and stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids...2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial...nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes. To target a given ...NGG PAM sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized...2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized...SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized...

...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse...mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit...

...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps...encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins, which... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing...in a precise manner. One of the first microbial opsins to be adopted as an optogenetic tool is the widely...anions into or out of the cell. When using these opsins as a tool to control neuronal activity, scientists...expression and localization. Many other types of proteins can also be controlled by light, generally by ...phytochromes, or light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as an optical ...

...commonly used adenovirus terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description...beta-gal Shuttle Test plasmid that contains β-gal and GFP; contains ∼10Kb genomic DNA stuffer Vogelstein...modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions into single...Collection of adenovirus shuttle vectors and strains Viral...Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids...database for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1...recombining shuttle plasmids; for use with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral A ...

... monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount...of membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to an antigen...pull out a protein of choice from a mix of other proteins, visualize a protein under a microscope, or detect...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots... Antibodies: Selecting and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology...to rapidly engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which secrete...

...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase...desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically ...

...enzyme-tagged preys. Ectodomain inserts: The inserts that encode the whole ectodomains of cell surface receptors... receptor and secreted proteins as soluble recombinant molecules. The proteins are typically used in systematic...microti blood stage proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm Mouse... Planck Institute Mitch Levesque University of Zürich Gavin Wright , Wellcome Sanger Institute Protocols... regions of cell surface receptor and secreted proteins as soluble recombinant molecules. Plasmid...in which the extracellular regions of receptor proteins are expressed as both monomeric baits and multimerised...NotI at the 5' end and AscI at the 3' end. These inserts can then be sub-cloned into a series of expression...