Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
Type
Blog Post
...time-consuming aspects of traditional cloning is the identification of clones that actually contain your insert...technology.
CcdB: A potent toxin...
The ccdB gene, located on the F sex factor plasmid of E. coli, is part...enzyme-mediated disruption of ccdB, leading to positive identification of desired clones.
...Becomes a powerful...which can then be used for downstream cloning applications. While these two strains ultimately perform ...