We narrowed to 772 results for: Dos

... us played with LEGO as a child (some of us still do), so we are used to the idea of general interfaces...

... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

...strand breaks (BLESS), revealed that these mutants do not cause off-target effects at unanticipated sites...

...Writing Scientific Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript ...

...application process? What types of writing feedback do you find most helpful? Have a great grant writing...

... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...

...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

...after knockdown of beta-catenin. Other gene targets do not require amputation to be visible. For example...

...  Listen to the CRY2clust podcast segment The Won Do Heo lab at the Korea Advanced Institute of Science...

...every C.elegans  neuron. Scientists are currently doing this using two approaches: single-cell RNA-sequencing...

...blurb you will use to introduce yourself and what you do. You might want to include your university and your...

...backbone. Purpose Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses...

...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here!  Cloning...

.... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the...

...Addgene.org Find Info on How to Quantify Your DNA Learn to Do a Bacterial Transformation Learn How to Perform a...

...study of heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics...

...share your protocols with other groups. What apps do you use to improve your lab productivity? As this...

...buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1-3...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...min until the agarose is completely dissolved (but do not overboil the solution, as some of the buffer ...to the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will...the DNA in your sample lanes. For more details on doing diagnostic digests and how to interpret them please...typically used to separate 5 - 500 bp fragments. How do you get better separation of bands? If you have similarly...