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Addgene

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We narrowed to 768 results for: Dos

Showing: 601 - 620 of 768 results

  1. Antibodies 101: Conjugation

    Type
    Blog Post
    ... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

  10. A Look at Addgene's QC Process

    Type
    Blog Post
    .... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

  11. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...

  13. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

  16. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the...

  20. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1-3...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...min until the agarose is completely dissolved (but do not overboil the solution, as some of the buffer ...to the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will...the DNA in your sample lanes. For more details on doing diagnostic digests and how to interpret them please...typically used to separate 5 - 500 bp fragments. How do you get better separation of bands? If you have similarly...
Showing: 601 - 620 of 768 results