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  1. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims...

  2. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...

  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...changes to a gene sequence can cause unexpected detrimental results to the protein. Codon optimization could...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...

  5. How to Be an Excellent Trainee

    Type
    Blog Post
    ...than any other one. Luckily, you can do a lot to contribute to the relationship dynamic and set yourself ...notes on general protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and ...

  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...vial of the desired cell line from a reputable distributor such as the American Type Culture Collection ...cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels...

  7. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...doing so, they identified the nucleotides that contribute to the instability of Spinach, and were able ...superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA." Nature methods 10.12...

  8. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...validated antibodies? Many companies that produce and distribute antibodies validate them in a variety of applications...

  9. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were developed...

  11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...sulfate proteoglycan (HSPG)3 AAV receptor (not strictly required)2 Human fibroblast growth factor ...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! References and resources References...

  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory Board member...target sites occur ~1 in every 8 nucleotides, restricting gRNAs to 5 – 65% of the protein coding region...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... activated tools, can control everything from electrical activity to gene expression, and even CRISPR ...2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202. https...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection...Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible...

  15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...all know that in the lab there are often little tricks that are essential for experiments but that nobody... not (Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the ...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... your time as they can result in spurious yet intriguing findings that fail to replicate. Plan experiments...

  17. Finding Your Science Policy Path

    Type
    Blog Post
    ...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

  20. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...
Showing: 581 - 600 of 882 results