...we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years...

...communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ion-based...

...interest into a bacterial cell or a host’s genome. To do this scientists commonly use phagemids, a DNA cloning...

...spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling split...

...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...

...Central PMCID: PMC5120861. While there is much work to do, these early pilot studies have demonstrated technical...

...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...

...strand breaks (BLESS), revealed that these mutants do not cause off-target effects at unanticipated sites...

...and Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand...

... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

...buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1-3...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...min until the agarose is completely dissolved (but do not overboil the solution, as some of the buffer ...to the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will...the DNA in your sample lanes. For more details on doing diagnostic digests and how to interpret them please...typically used to separate 5 - 500 bp fragments. How do you get better separation of bands? If you have similarly...

...H134R) both reach the brain, but individual neurons do not express as many copies of the ChRs as compared...

... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...

...RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help answer?...

...References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis Ferreira, V. & Videira, P. A. Immunological ...

...colony of genetically engineered mice, what can you do? In this post, we’ll cover why and how to cross mice...

...: A New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in...

...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...