Addgene

We narrowed to 646 results for: CHI

Showing: 581 - 600 of 646 results

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Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

...know the catalog number of the plasmid you are searching for, this is the quickest and easiest way to find...
Hot Plasmids: Winter 2025

Type

Blog Post

...inherited the genomic edit but not the editing machinery. Together, these advances have the potential to...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...vectors can be made at high titers equal to those achieved with lentivirus but offer an improved safety profile...
Antibodies 101: Flow Compensation

Type

Blog Post

... Inputting compensation values differs between machines or the program you’re using, but usually you can...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

... of the post! Speed: Your grant deadline is approaching or your advisor is demanding results. If you’...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

...dengue for many years. Since more than a thousand children died of malaria today, let's not risk a potential...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know the species that...
Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...
DNA Purification Without a Kit

Type

Blog Post

...method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather...
MXS Chaining

Type

Blog Post

... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...
Lentiviral Vector Uses and Overview

Type

Blog Post

...Second, Tat is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. ...
Plasmids 101: Choosing an Antibiotic Resistance Gene

Type

Blog Post

..., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly...
Viral Vectors 101: The Retroviral Lifecycle

Type

Blog Post

...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...
Negative Can Be Positive: Open AAV Data with Addgene

Type

Blog Post

...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...Addgenies from wasting hours of valuable time searching for samples and keeps us from discarding tubes...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed structure...

Showing: 581 - 600 of 646 results

Results per page:

Hot Plasmids and Viral Preps - May 2021

Anatomy of a Plasmid Page at Addgene

Hot Plasmids: Winter 2025

The AAVantages of AAV in CRISPR Screens

Antibodies 101: Flow Compensation

Plasmids 101: 5 factors to help you choose the right cloning method

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Tips for Using BLAST to Verify Plasmids

Transferable Skills Guide: Identifying Your Transferable Skills

DNA Purification Without a Kit

MXS Chaining

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Lentiviral Vector Uses and Overview

Plasmids 101: Choosing an Antibiotic Resistance Gene

Viral Vectors 101: The Retroviral Lifecycle

Negative Can Be Positive: Open AAV Data with Addgene

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Cpf1: A New Tool for CRISPR Genome Editing

Tips from the Repository Trenches: Using Barcodes to Track Samples

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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