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Showing: 41 - 60 of 1028 results

  1. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...packaged strains, which are shipped as bacterial stabs at room temperature. Individual plasmids can be ...each plasmid page and will be shipped as bacterial stabs. Add to Cart $ 350 USD + shipping Available to academics...Contents The following items will be shipped as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4...

  2. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... director for more information. Why MDH? MDH is stable, inexpensive to assay, and easy to purify using...institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression...Link opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching...

  3. EXtracellular Plasmid RESource (EXPRESs) Consortium

    Type
    Collection
    ...: Basic AVEXIS : A list of all you need to get started with AVEXIS, including positive and negative controls...receptors Human erythrocyte Babesia microti blood stage proteins P. falciparum sporozoite cell surface and...invasion by Plasmodium falciparum. Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, Uchikawa...

  4. mTOR Pathway

    Type
    Collection
    ...Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic... Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini...

  5. AAVED

    Type
    Collection
    ...Co-Founder and CSO, Addgene Organizing Committee: Dr. Constance Cepko, Harvard Medical School Dr. Melina Fan, ..., Dr. Meghan Rego, Addgene Confirmed Speakers Constance Cepko Harvard Medical School Ali Cetin Allen Institute...technology or its application? What are some current obstacles that researchers face right now (e.g., not enough...

  6. Qi Lab CRISPR Page

    Type
    Collection
    ...dcas9 gene on a p15A vector with chloramphenicol resistance. The second plasmid ( pgRNA_bacteria ) contains...expression cassette on a ColE1 vector with ampicillin resistance, wherein the N20 can be custom designed to target...gene 46919 pMLS-SV40-EGFP Target EGFP gene that is stably integrated into HEK293 genome 46922 pSNR52-sgTEF1...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Plasmids rsTagRFP 440 / 567 585 / 585 0.02 / 4.0 6.6 43 min Prone to dimerization pBAD/HisD-rsTagRFP - Bacterial...Structure describes the monomeric or oligomeric state of the protein. Many FPs are monomers at low concentration...pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish...

  8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...tested at the beginning. Variability source Standardization parameters Animal Strain, age, sex, housing...conditions (individually versus in groups), health status, diet, cage enrichment, stress level, origin (vendor...Alternatively, for larger brain areas, repeated staggered injections may be considered. Timing: High expression...situations such as learning and memory or emotional states, for example anxiety or depression. In upcoming...

  9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...expression stable regardless of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters...keep gene expression stable regardless of copy number, the team made use of stabilized promoters. In contrast...and keeping its expression level constant. In the case of the stabilized promoters from Segall-Shapiro et...gene expression also remained stable regardless of plasmid copy number. Stable gene expression whether growing... were expressed. What’s the use of stable gene expression? Constant gene expression can enable more confidence... to unregulated promoters that are always on, stabilized promoters incorporate feedback loops such as ...with copy numbers ranging from 3-100 per cell, a stabilized promoter buffered against the effects of copy...

  10. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express...

  11. CRISPR Plasmids - Plants

    Type
    Collection
    ...to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible...pyogenes Stupar pBUE411 OsU3 yes, cut S. pyogenes Basta Chen pHUE411 OsU3 yes, cut S. pyogenes Hyg Chen ...pyogenes Hygro Kim pBAtC U6 AarI yes, cut S. pyogenes Basta Kim pHEE401 U6-26p BsaI yes, cut S. pyogenes Hygro...

  12. Open Enzyme Collection

    Type
    Collection
    ...polymerase 165501 pOpen-GBDpol Pyrococcus sp. Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees...P45 165572 pOpen-GBDpol (exo) Pyrococcus sp. Heat-Stable (exo-) DNA Polymerase 165573 pOpen-phi29pol phi29...DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579 pOpen-dromedNK...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...in need of reference spectra or phosphopeptide standards for quantitative assessments. The mode #1 phosphosite...lactamase S68TAG Plasmid 69118 E17TAG GFP zeo resistance Strain 34929 BL21ΔserB Strain 52055 EcAR7 Plasmid...

  14. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other/Tribolium castaneum BsaI none...Hygro Yang pBUE411 62200 Plant yes, cut S. pyogenes Basta Chen pKSE401 62202 Plant yes, cut S. pyogenes Kan...Kim pBAtC 78097 Plant AarI yes, cut S. pyogenes Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ...gene of interest rtTA or TetR expression plasmid Stable cell lines can be made that continuously express...lines Tet-On Advanced; plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434 pMA2640 ...underlying expression of Tn10 encoded tetracycline resistance. Hillen W & Berens C. Annu Rev Microbiol. 1994...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...barrier integrity, and using CRISPR screens to understand more globally how neurons function. They are ...and death of dopamine-producing neurons in the substantia nigra, one of the movement control centers in...Study the C terminal domain of TDP-43 to better understand its function and pathology. Mohanty et al. Proc...

  17. Synthetic Biology - Strains

    Type
    Collection
    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back to Top Do you have suggestions...

  18. KLF Research Plasmids

    Type
    Collection
    ...efforts within the KLF community have lead to the establishment of this shared resource on Addgene. If you have...with Addgene or email [email protected] to get started. Genes KLF gene pages with information on available...

  20. Cancer Research Plasmids and Resources

    Type
    Collection
    ...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris...
Showing: 41 - 60 of 1028 results