...protein The MLV Gag protein fused to a Flag-tagged SpCas9 is incorporated into Nanoblades, just like the ...these variants are fused to the MLV Gag in place of SpCas9. gRNA A gRNA targeting a DNA sequence of interest...

...Enhance CRISPR Targeting Specificity with eSpCas9 and SpCas9-HF1 See How Cpf1 Stacks Up to Cas9 and NgAgo...

...multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software...configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency (percentage...

... for reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options...

...target sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping... From the lab of Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used ...

...compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other benefits...

...2 and 6 kb smaller than commercially available pCAMBIA and pKYLX vectors, respectively, making it ideal...

...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...

...nickase experiment Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation...

...The PAM Requirement and Expanding CRISPR Beyond SpCas9 The closer your edit is to the PAM site, the higher...

...collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO...

...887 amino acids in length, NgAgo is shorter than SpCas9 (1368 aa), SaCas9 (1053 aa), or Cpf1 (1228 or 1307...

...different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to...

...promoter directs broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs...

...pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3'...NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3...engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular...no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...RuvC groove and the non-target DNA strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone...your desired genes for modification. Engineered SpCas9 enzymes with altered PAM specificities are available...

...with these modified gRNAs is comparable to that of SpCas9. The group also confirmed that the Cas12f1 and ...

...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

... Council of Advisors on Science and Technology [PCAST] (2012). Engage to Excel: Producing One Million ...

...constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog...