... to keep in mind, but likely will not have a significant impact on your data. You are trying to generate...selectively sequenced to determine if there are significant editing events, serving as a proxy for total ... to effectively control for off-targets and significantly decrease the probability of artifacts. Off-target...

...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

...published Aug 16, 2016 and last updated Aug 6, 2020 by Jennifer Tsang. When we talk about CRISPR applications,...mixed population of edits, ABEs do not display significant A to non-G conversion at target loci. The removal...Has both cytosine and adenine base editing Jennifer Tsang contributed to updating this article. References...

...homology region. Hairpins in this region can significantly reduce the efficiency of two homologous ends...gel to check for size and yield. If there are significant amounts of undesired product, gel-purify DNA ...

...welcome suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

... have your first cold of the season like I do, sniffle) ourselves back into our regular post-holiday routines...Researchers in Taiwan showed that a laughter program significantly decreased immunological markers of stress in...

...MCDB 🎄🎅🎁🐸🐸🐸🐸 pic.twitter.com/2MPLawgNQl — Jennifer Landino (@jenelandino) December 3, 2019 Hope...  Jennifer Tsang...

...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...

...there's a pretty good reason for that. Lately, significant advances in the understanding and application... work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna and Eva Nogales presented the first detailed...

...a bioinformatics and a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors... Jennifer Tsang...

...that lab and interacting with the lab members.”- Jennifer Tsang, Science Communications and Marketing Coordinator... Jennifer Tsang...

...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... Jennifer Tsang...

...active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX... Jennifer Tsang...

...discovers of CRISPR/Cas9 genome engineering, Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier. I was joined...especially excited to attend this event because Jennifer did her graduate work one floor above mine in ...

...IgGs and your primary antibody, resulting in significant background staining (Figure 1A). Not all tissues...Figure 2B) Some labs have found that you can significantly decrease background staining by mixing your ...

...excitation maximum 399 nm) because it does not significantly excite at 400 nm while EGFP does. Find mWasabi... Jennifer Tsang...

...of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity (see ...