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Addgene
Showing: 41 - 60 of 138 results

  2. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...uniquely positioned to help research institutions maximize their increasingly limited resources: Preserving...resources become even more valuable as labs look to maximize productivity and efficiency with fewer resources...

  4. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...deamination. They generated these editors using the BE4max architecture. The new editors reported in this ...average on-target DNA-editing levels relative to BE4max.  Read the paper in Nature Biotechnology Find ...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...amino acid sequence of the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and...disease.  Read the paper in Cell Find PE4, PE5, and PEmax plasmids Precise genomic deletions In PRIME-del...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...through several rounds of stripping and reprobing, maximize your signal by starting with the least-abundant...Immediately after transfer, dry the membrane to maximize protein retention. Following this step, most PVDF...

  11. Hot Plasmids: Winter 2025

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    Blog Post
    ...1: Left: recombined prime editing tools PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA...

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...growth time, temperature, and shaking speed to maximize cell density and plasmid yield as these factors...need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time should be...

  13. Great Results Start with Great Standard Curves

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    Blog Post
    ...dilution series of the standard and the minimum and maximum detection limits of the instrument being used to...specificity, especially as it approaches the minimum and maximum thresholds. If the measurements for the unknown...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    ...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ...plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits ...

  16. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...s illumination (see Figure 2). H describes the maximum signal strength, while W results from the time ... The resulting histograms is described through maximum current (H), the time the cell needed to pass through...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional allele...Selection of gRNAs for an experiment needs to balance maximizing on-target activity while minimizing off-target...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...population level, but each individual cell only has a maximum of one gene knocked out. In this genetically heterogeneous...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...
Showing: 41 - 60 of 138 results