We narrowed to 167 results for: ipp

1. Scientists Map the SARS-CoV-2-Human Interaction Network

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   ...line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at UCSF. Our team members...Foussard, and Dave Gordon) have been quite busy shipping and sending plasmids to all who request them -...

2. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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   ...-assembling split GFP (GFP1-9 and GFP10-11) by flipping a single β-strand of GFP, hence the name FlipGFP...Designing a green fluorogenic protease reporter by flipping a beta strand of GFP for imaging apoptosis in ...

3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ... anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged 14 days post-transfection... the screen to understand mouse cortical and hippocampal neural microcircuits. “In the longer term, this...

4. Celebrating 15 Years of Scientific Sharing

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   ... despite the evolving export/import rules. Our shipping and lab teams continue to keep up with the demand...have confidence that from Business Development to Shipping we will execute any new projects with our usual...

5. Addgene's Tips for Plasmid Quality Control

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   ... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...volume and concentration, and not all providers are equipped to perform Sanger sequencing from glycerol stocks...

6. Plasmids 101: Cre-lox

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   ...conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) site being...

7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   ... from their assay to Addgene.  Drew Endy’s and Philippa Marrack’s labs also deposited plasmids for producing...Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020...

8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...

9. Distribution to Industry

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   ...MTA documents, your order will be processed and shipped. Industry Distribution Policy A portion of Addgene...

10. Common Injection Routes in Mice

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    ...Restraint: scruffing Location: throat Gauge (bulb-tipped feeding tube): 18–20 Volume: up to 10 ml/kg Skill...Beginner Oral gavage Scruffing Throat 18–20 (bulb tipped) up to 10 mL/kg Intermediate Resources and...

11. mTOR Pathway

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    ... B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex...

12. Tips for arabidopsis transformation

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    ... Growing the transformed agrobacterium culture Dipping your plant’s flowers in the agrobacterium culture...resuspend them in transformation media. If you are dipping your plants, put the culture into a container that...

13. DNA Purification Without a Kit

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    Blog Post

    ...and ball bearings. Then a cellulose dipstick is dipped into this mixture to absorb the nucleic acids. ..., purified total nucleic acids are released by dipping the cellulose directly into a PCR buffer, where...

14. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ... editing enzyme (“Editase”), ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated...RNA in each of three neuronal subtypes of the hippocampus. This technology has formed the basis of the ...

15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ... the Clevers lab developed the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used...PMID: 30454553. PubMed Central PMCID: PMC6245733. Snippert, Hugo J., et al. "Intestinal crypt homeostasis...

16. Harnessing Bacterial Toxins for Allelic Exchange

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    ...performed the following modifications to pDS132 (Philippe et al., 2004): Replaced the sacB cassette with...PMID: 29760772. PubMed Central PMCID: PMC5946454. Philippe, Nadège, et al. "Improvement of pCVD442, a suicide...

17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ..., including R26R-Confetti described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described...between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der Flier LG, Sato T, van Es JH, van den...

18. Important Considerations When Using AAVs

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    ...Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2 Hippocampus AAV9>AAV5>AAV8>AAV1>AAV6>AAV2 Auditory Cortex...upon which section you’re looking at (striatum, hippocampus and cortex) within the mouse brain (4). An example...

19. How to Deposit Your Plasmids with Addgene

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    ...Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially...

20. COVID-19 Resources

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    ...transcriptase expression plasmids from the Drew Endy and Philippa Marrack Labs. Point-of-care testing for COVID-...