Skip to main content
Addgene
Showing: 41 - 60 of 61 results

  5. Synthetic Biology - Overview

    Type
    Collection
    ...Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

  6. Adenovirus Plasmids

    Type
    Collection
    ...pEZYshuttle Shuttle Modified pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang...

  7. Cloning

    Type
    Guide
    ...Back to Top Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway... Gateway® cloning is a recombination based cloning method. The benefit of Gateway® is that moving a piece...cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and recombination...Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning...fragment must first be amplified with specific Gateway attB1 and attB2 sites attached to the 5’ and 3’...sequence. This fragment can then be cloned into a gateway donor plasmid which contains compatible attP sites...it can be rapidly shuttled into any compatible Gateway® Destination vector, which contain attR sites via...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex polycistronic...plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly of...a single T-DNA binary vector of choice through Gateway recombination. Qi Multiplex Conditional Mutagenesis...

  12. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest...

  14. Ras Pathway

    Type
    Collection
    ...vectors from the Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit...

  15. Lentiviral Guide

    Type
    Guide
    ...restriction sites but compatible with the Gateway® cloning system? Gateway® compatible vectors use recombination...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB survival...Invitrogen's website for more information on the Gateway® cloning system. What safety concerns surround ...

  16. Sequencing Primers

    Type
    Guide
    ...GCATGATGACCACCGATATG 3' end of Gateway cassette, forward primer GW-5' AATCTCGCCGGATCCTAACT 5' end of Gateway cassette, reverse...

  18. Molecular Biology Reference

    Type
    Guide
    ...fragment. Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you ...

  19. Cre-lox system

    Type
    Collection
    ...pENTR/D_creERt2 Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140...
Showing: 41 - 60 of 61 results