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  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient experimental use. The plasmids...summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid...

  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible...

  4. Brain Armamentarium

    Type
    Collection
    ...yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...PHPeB AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) Expression...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...protocol. Both plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a...

  6. Control AAV Preps

    Type
    Collection
    ...Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent ...

  7. Molecular Biology Reference

    Type
    Guide
    ...prokaryotes. Common Epitope Tags Tag Amino Acid Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL ...

  8. Bacterial Expression Systems

    Type
    Collection
    ...Various Plasmids T7-lacO (lactose/IPTG inducible) Flag, His, Strep, mOCR, MBP, GFP, with and without SUMO...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...there are many types of epitope tags (eg. HA, MYC, Flag), they can be deleterious and cause aberrant protein...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

  11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision...synthetic intron, and only the fluorescent protein and 3xFLAG-tag sequences are inserted into the genome. ...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...

  12. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...by Paix et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered...digests of PCR products used to identify a single 2xFLAG knock-in by direct screening. The center images...mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes into other, non-linked loci; pha-1(e2123...

  13. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...

  18. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) AAV-PHPeB ...
Showing: 41 - 60 of 76 results