...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP...Anti-FLAG frankenbody (FB) construction from the complementarity determining region of an anti-FLAG scFv...targets with nuclear (top, 4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA frankenbodies...here.  Here's what you'll find in this post: Anti-FLAG Frankenbodies for modular imaging of cellular targets...for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular imaging of cellular targets...Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, modular tool for labeling...

...pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype version: pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt...you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene If ...insert you would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert ...

...their antibodies along with some pros and cons. FLAG FLAG is a synthetically derived 8 amino acid tag (DYKDDDDK...tags, FLAG is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag...DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s termini to increase...success rates across all applications. You can find FLAG antibodies on Addgene’s catalog including a mouse...

...Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we know and love, the FLAG tag (amino...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing...plan to purify or image a recombinant protein, the FLAG tag has proven itself a good option for essentially... collection includes over 10,000 plasmids with a FLAG tag (!), but did you know that we distribute ready-to-use... (original mouse Fab region with rabbit Fc) anti-FLAG antibody, both of which are also available in trial...

...second plasmid inserts a small epitope tag called FLAG at the end of the transcription factor. It also ... cells with the tagged transcription factor. The FLAG antibody has been well characterized and the system...protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small—20 amino acids—so we don’... selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have... flag...

...we need new epitope tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations...effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not... observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental effects on...

...protein may be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have ...elution steps use very moderate buffer conditions FLAG DYKDDDD or DYKDDDDK or DYKDDDK 1 Affinity and Purification...friend rather your enemy. Two common tags (SUMO and FLAG) are cleaved by specific proteases without requiring...manner as it would have in the context of a cell. FLAG tags can be cleaved by enterokinase, which recognizes...

...our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the ... a mammalian selection cassette (e.g. 1435 pSG5L Flag HA). You can also upgrade your basic mammalian expression...

... on its C-terminus, SpCas9 is also fused to a 3X FLAG tag at its N-terminus. This tag can be used to test...

...different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry...

...with David Goldstein’s lab, will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional...

...designed for technical writing), it’s great at flagging passive voice, overuse of adverbs, and paragraphs...

...resulted in the development of a new plasmid, pCMV14-3X-Flag-SARS-CoV-2 S, which is now easily available through...

...are available for tagging with epitope tags (e.g. FLAG and STREP) and auxin-inducible degrons (AID). Further...

...Gag-Cas9 fusion protein The MLV Gag protein fused to a Flag-tagged SpCas9 is incorporated into Nanoblades, just...

...finer points of the protocols. Are there any red flags about how the work was performed that could influence...

...offers C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin or ...

...threshold at >100 hits - immediately raises a red flag. For example, AAV-68544 contained 220 hits to the...

...Zhang et al. shows just that - using MMEJ to insert FLAG tags into the genome of the pathogenic fungus Aspergillus...

...et al. "Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells...cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their genomic... In addition to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have...Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9...chromatin or DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA...