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Showing: 41 - 60 of 155 results
  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., calmodulin or troponin C). Their fluorescent readouts are used to monitor calcium concentrations and...sensor option if you have the setup necessary for readout.   We’ve got a live one (calcium sensors...
  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...happens (this time, Poisson statistics), and the readout gives an absolute value of DNA concentration. By.... PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, ...
  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired...
  4. Luciferase Plasmids

    Type
    Collection
    ...Plasmids from 2016 blog post. LuTHy plasmids (Double‐Readout Lu minescence‐based T wo‐ Hy brid) : Combines in...
  5. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ....1038/s41597-021-00981-0 Wright, J. F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., ...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A....
  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...number of bands present on the strip. This type of readout allows for nucleic acid detection almost anywhere...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...
  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...escape a threat. Locomotion can thus be used as a readout for associated emotional phenomena like stress ...2018). Locomotion can also be used as behavioral readout to study certain forms of drug addiction. When ...
  8. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...approach can be incorporated into a lateral-flow readout slip that allows rapid detection.  Highlights: ...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...
  9. Antibodies 101: Validation

    Type
    Blog Post
    ...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL...
  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...targeted cells. Depending on the cells used and the readout, this could be a major limitation. An alternative...custom CRISPR screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by ...
  11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...have the proper controls or a proper behavioral readout that allows you to interpret the results of your... high quality stereotaxic frame with a digital readout. The advantage is you can “zero” your coordinates...
  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...inducible gene expression Brilliant technologies adopt basic biological concepts and employ them in creative...copies of targeting sequences. Scientists have adopted and employed this concept in both imaging and inducible...observed at a later time via DNA sequencing or other readout. Cellular memory is accomplished by the use of ...
  13. Technical Design of a Western Blot

    Type
    Blog Post
    ...easy to source, making it easy to switch from one readout method to another or change primary antibodies ...this truly does provide a relative, not absolute, readout. Normalization Overall protein expression varies...
  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...., Greenbaum, A., Ravi, N., Wu, W.-L., Sánchez-Guardado, L., Lois, C., Mazmanian, S.K., Deverman, B.E....PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski...
  15. Antibody Guide

    Type
    Collection
    ...Sample preparation, signaling excitation, and assay readout vary widely depending on individual assay conditions...
  16. CRISPR Guide Update

    Type
    Blog Post
    ...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing the...
  17. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción, Addgene contrató...
Showing: 41 - 60 of 155 results