...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes of...Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast...

... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase... window, and are thus well suited to directed evolution applications. Examples of these base editing systems...

...experiments such as immunoprecipitation and super-resolution microscopy. Two commonly used plasmid-based antibody...simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnol. 2006...

...response data, list of antibodies and recommended dilutions, etc.), novel applications, or any other useful...successfully adopt these tools. Thank you for your contributions and your commitment to open science! Please ...

...they induce. Some (oncolytic) adenoviruses are utilized for cancer therapy because of their ability to...and envelope used. Please consult with your institution’s Biosafety Committee before beginning virus work...

...enhance expression of the message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden... pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built into this vector....

...on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours),...cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site...system for regulated gene expression through viral evolution. Zhou X, Vink M, Klaver B, Berkhout B, Das AT...

...lab recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from...The Zhang lab uses around 50ul of the extraction solution, runs the protocol, then uses around 2-4ul in ...

...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); ...genetic knockouts, gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances ...

... an excellent unlimited stem cell source for therapeutic use. Since 2006, scientists have developed many...reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression of key factors....

...Vector Core will produce viral-based vectors for distribution via Addgene. For a full list of Addgene's viral... The following vectors are now available for distribution at Addgene: Penn ID Addgene ID Name Function...

...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and ...1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise...

...engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research...

... iNDI iPSC line generation, phenotyping, and distribution efforts. Plasmids The following plasmids can...

...extracellular protein interaction network and its resolution by spatiotemporal expression profiling. Martin...

... Depositing Principal Investigators (PIs) PI Institution NETRF Project Title Hans Clevers Hubrecht Institute...

... ( Sander et al., Nat Biotechnol. 2011 ). REAL utilizes a set of 28 plasmids each encoding a single TALE...

... In-stock AAV preps are distributed as 100 µL solutions of purified viral particles that are suitable ...

...modified from a bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page...

...abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts like ...