...transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Zhou, D., Lin, S. W., Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl...

... the past few years, scientists have created an array of base editing reporters that can help you do just...successfully used GO to induce the translation of an array of different reporters including mScarlet-I, luciferase...initiate protein expression, it can be used for an array of detectable proteins including fluorescent proteins...

...Institute Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets...DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is ... can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with...

...three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme...of these platforms assemble TAL effector repeat arrays on a single architectural framework and the DNAs... are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs which...

... has become an ideal model organism to study an array of topics including development, genetics, and the... A huge advantage of using the fruit fly is the array of genetic tools, such as the GAL4/UAS and LexA ...organisms. Worms have been used to investigate an array of biological systems and processes including the...

...2009). Rodents have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016...biology tools to generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed...in research (Krämer, 2015). As a result, a vast array of molecular tools, such as gene manipulation and...

...so that at least one might be useful in a broad array of pathogenic Proteobacteria. We placed the toxin..., Shigella flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions...development of allelic exchange vectors will produce an array of great options, and continue to improve the efficiency...

...pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11...reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12...be used to visualize translocations, genome rearrangements and cancer-associated chromosome shattering...subpopulation of mitochondria is selectively labeled by irradiating the photoactivatable FP, then the overlap between... MYC, Flag), they can be deleterious and cause aberrant protein folding, expression, or even affect cell...

...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular... 2017 Nov 10;8(1):1412. Justin Taraska , David Perrais pH Sensor for intracellular chloride and pH (ClopHensor...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...

...PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF 125.6 KB Marraffini Bacteria: pCRISPR...pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9...

...209552 Knockout Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid...Library 130630 Knockout Human Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR...Library 123361 Knockout Mouse Chen 3rd 4 11,934 arrays Mouse SLC Knockout Library 141436 Knockout Mouse...

... depositors for sharing your science with us! Corralling and Cornering the Goldilocks Antibody: Guest ...drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen...

...uncharacterized genes that were proximal to a CRISPR array and the universal CRISPR integrase, cas1. They discovered...the cas14 gene together with the adjacent CRISPR array and intergenic regions containing the putative tracrRNA...

...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...

...Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your work The part...programmed to appreciate and understand. We build narratives around the self, too — when talking to friends...

...ahead of time included contacting YNP staff to arrange permits to visit places not accessible to the public...and rigorous assembly. There, they found a CRISPR array in the resulting Ignavibacteriae phylum genome. ...

...supporting your research, Addgene is creating an array of educational materials to accompany our antibody..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of...

...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well ...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

...the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear processes...

...and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as the REAL...restriction sites used for ligation into longer arrays. These 28 plasmids are available individually or...