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We narrowed to 113 results for: CHL

Showing: 41 - 60 of 113 results

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Plasmids 101: Antibiotic Resistance Genes

Type

Blog Post

...elongation step) Bacteriostatic 10 ug/mL Chloramphenicol Binds 50S ribosomal subunit; inhibits peptidyl...
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

Type

Blog Post

...References and Resources References Romano E, Baumschlager A, Akmeriç EB, Palanisamy N, Houmani M, Schmidt...
Plasmids 101: Origin of Replication

Type

Blog Post

...amplifying more copies with the addition of chloramphenicol – make sure your growth conditions aren't working...
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Type

Blog Post

...sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled...
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

Type

Blog Post

...genomic locus: Caspex Article contributed by Susanna Bachle Listen to the Caspex podcast segment Steven Carr...
Viral Vectors 101: Parts of the AAV Transfer Plasmid

Type

Blog Post

...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

Type

Blog Post

...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

...optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting channelrhodopsins (ChR) are ion...
Summer SciComm Series: A PhD in Science Communication

Type

Blog Post

...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all...
Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

...types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively charged iodine...
R Bodies: Membrane-Rupturing Microscopic Tools

Type

Blog Post

...Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive...
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

Type

Blog Post

... Susanna Bachle...
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Type

Blog Post

...is in citizen science and behavioral ecology of cichlid fishes. Find David online at https://orcid.org/...
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Type

Blog Post

...active LacZ tetramer. This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

Type

Blog Post

... Susanna Bachle...
Multiplex Genome Editing with CRISPR-Cpf1

Type

Blog Post

...Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P., Zhang, F. (2015). Cpf1 Is a Single RNA-Guided...
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Type

Blog Post

...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...
Antibodies 101: ChIP

Type

Blog Post

... with a PCR purification kit or with a phenol-chloroform extraction. Fig. 1: Schematic overview...
Addgene's Tips for Plasmid Quality Control

Type

Blog Post

...expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save these ...
Working with Nuclear Receptors

Type

Blog Post

...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...

Showing: 41 - 60 of 113 results

Results per page:

Plasmids 101: Antibiotic Resistance Genes

A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

Plasmids 101: Origin of Replication

Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

Viral Vectors 101: Parts of the AAV Transfer Plasmid

Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Summer SciComm Series: A PhD in Science Communication

Plasmids 101: Gram Negative and Positive Bacteria

R Bodies: Membrane-Rupturing Microscopic Tools

Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

Multiplex Genome Editing with CRISPR-Cpf1

Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Antibodies 101: ChIP

Addgene's Tips for Plasmid Quality Control

Working with Nuclear Receptors

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