... using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to ...material. Then, the homogenate was loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours... protocol with guanidinium thiocyanate-phenol-chloroform. In this method, samples are still homogenized...ultracentrifugation!), the layers of phenol and chloroform separate, and RNA is retained in the top, aqueous...centrifugation and the supernatant is extracted with chloroform as in the above no-kit method.  Researchers have... trick is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but...Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159...

...conferences have followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended virtual conferences...walk 20 minutes between one session to another,” Bachle says. “Now, the physical location didn’t matter... asking in front of many people is lower,” says Bachle. (For presenters, there’s also less stage fright... meeting, I received a lot of LinkedIn invites.”Bachle pointed out that networking at virtual conferences...conference, you really have to make space for it,” says Bachle. “On the flip side, you can attend conferences ...

...once, vomit, and never do it again. Cichlids (Scott Juntti) Cichlids are 2,000 fish species that evolved...’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes and Mormyridae...filter. Development is direct in this species, and hatchlings are around 1mm long pic.twitter.com/sIIoIsIFd1...evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the...

...General protein expression BL21 (DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to reduce...Expression of toxic proteins BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression...Expression of insoluble proteins  Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal” translation; contains...Expression of eukaryotic proteins Lemo21 (DE3)* Chloramphenicol (pLemo) Rhamnose-tunable T7 RNAP expression...T7 expression. The pLys plasmid contains a chloramphenicol resistance cassette for positive selection ...

...stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the...wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and relies ...algae host for reproduction. Snail parasite Leucochloridium is a parasitic worm that infects a snail’s ... the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723...

...supercoiled, open circular, linearized, etc.) (Schleef et al., 2006; Holovics et al., 2010; Cook et al...PMID: 19763938. Maucksch C, Bohla A, Hoffmann F, Schleef M, Aneja MK, Elfinger M, Hartl D, Rudolph C. Transgene...s12033-008-9082-3. Epub 2008 Jul 8. PMID: 18607781. Schleef M, Baier R, Walther W, Michel ML, Schmeer M. Long-term...

...lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2 (AAV9), which contains a biosensor that...AAV9 Biosensor Tian New viral vector pAAV_hSynapsin_psychLight2 AAV9 Biosensor Tian New viral vector ...

...said to be hyperpolarized. The chloride pump NpHR brings in negative chloride ions which hyperpolarize the...

...mapping and design (Figure 1). Benchling: While you might think of Benchling as an electronic lab notebook...

...helveticum (ShChR), Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas oogama (CoChR...

... a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose...galactose and an insoluble blue pigment (4-chloro-3-brom-indigo). So far, so good, but how does this help us...

...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion...

...   Figure 2: Image from http://teachline.ls.huji.ac.il/72682/tutorials/gateway/introduction.html...OpenWetWare's CcdB Page Gateway Cloning Page at Techline ...

... of a common laboratory chemical, guanidine hydrochloride, as a potential treatment for a large number...be rescued by supplementation with guanidine hydrochloride (GdnHCl). Rescue occurs through guanidinium ...

.... The reaction is stopped by the addition of hydrochloric acid (for more details on this, see below). ...-Tetramethylbenzidine (TMB). The addition of hydrochloric acid, which stops the reaction, turns the solution...

...increase transduction efficiency by 1.5-fold (Stockschläder et al., 1999). 5. Check your packaging cells... (2002): 159-172. PubMed PMID: 12050007. 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced...

... were able to reconstruct a cleared guinea pig cochlea in 3D. The basic configuration of the instrument...Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten. Leipzig: S. Hierzel...

...on gene transfer, from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. ...in plants because of the auto-fluorescence of chlorophyll, so we looked at newer fluorescent proteins that...

...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine...

...post. References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ...