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Showing: 41 - 60 of 646 results

  1. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...-Puro 73039 Expresses Nluc and copGFP from the chicken beta-actin CMV enhancer pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro... the EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ...polyA pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA...

  2. Viral Production

    Type
    Collection
    ...ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering by the...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... in: A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation... in: Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon ... described in: Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  5. AAV ddPCR Titration

    Type
    Protocol
    ...gently touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well...nothing falls into the plate. Leave dilutions on the chilled 96-well freezer block in the BSC until ready to...Prepare the Master Mix Place a ddPCR plate onto a chilled 96-well freezer block and set aside in the droplet...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 20 µL of the master ...aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol...

  6. Luciferase Plasmids

    Type
    Collection
    ...sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common choice in assays... of renilla luciferase Reuben Shaw 48688 pUltra-Chili-Luc Firefly Ubc Lentiviral expression of firefly... Mammalian expression of firefly luciferase Ming-Chih Lai 115366 p CIneo-RL Renilla CMV Mammalian expression...

  7. CRISPR Library Amplification

    Type
    Protocol
    ...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to ...Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent...ice for 15-20 minutes or until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer...kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves...sterile LB at 4 ℃. Day 2 (morning) Before beginning, prechill at least four conical tubes on ice and ensure ...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ... order primers that are 60 bp long, with 30 bp matching the end of the adjacent fragment and 30 bp annealing...the assembled parts. Tips and Troubleshooting “Stitching” fragments together using oligos When you need...Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers to include...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

  9. Lentivirus ddPCR Titration

    Type
    Protocol
    ... gently touching the screen. Preparation of the Master Mix Place a ddPCR plate onto a chilled 96-well ...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 16 µL of the master ...pipetting the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the...

  10. Synthetic Biology - Overview

    Type
    Collection
    ...Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological Parts...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

  11. Chemogenetics AAV Preps

    Type
    Collection
    ...none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D...

  12. Qi Lab CRISPR Page

    Type
    Collection
    ...CRISPRi), works independently of host cellular machineries, requiring only a nuclease-deficient Cas9 (dCas9...binding specificity is determined jointly by 20-bp matching region on the sgRNA and a short DNA motif (protospacer...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013...

  14. Viral Vectors

    Type
    Collection
    ...scientific review of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid Biosafety...Au S, Panté N. How viruses access the nucleus. Biochim Biophys Acta. 2011 Sep;1813(9):1634-45. doi: 10.1016...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...using streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct...

  16. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...strategy relies on cellular delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double...of PCR products. Finney M, Nisson PE, Rashtchian A. Current Protocols in Molecular Biology. 2001...

  18. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the sample... that does not contain antibiotics. h. Without touching the sides of the dish, gently add DNA:FuGENE® ...PubMed . Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Zufferey R et...

  19. Bacterial Expression Systems

    Type
    Collection
    ...protein reporter genes in Gram-negative bacteria. Hochschild Bacterial Two-Hybrid System Various Plasmids ...with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid assay using pBRα-β flap ...

  20. Plasmids for Stem Cell Research

    Type
    Collection
    ... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...
Showing: 41 - 60 of 646 results