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  1. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. Are you...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. His interests...

  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ... editing enzyme (“Editase”), ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated...RNA in each of three neuronal subtypes of the hippocampus. This technology has formed the basis of the ...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...brain. The Allen Institute and the Janelia Research Campus in the USA have used AAV1 and AAV2 serotypes in...visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons which are known to be notoriously...

  5. Retrograde AAV viral preps

    Type
    Collection
    ...Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m GCaMP7f GCaMP7s GCaMP7b GCaMP6f GCaMP6s GECO Other Biosensors...pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium...-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn GCaMP8m Calcium...AAV-hSyn-Soma-jGCaMP8m Syn Soma-GCaMP8m Calcium sensor Fyhn 169258 AAV-hSyn-Soma-jGCaMP8f Syn Soma-GCaMP8f Calcium...-mDlx-jGCaMP8s-WPRE Dlx GCaMP8s Calcium sensor Looger 176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium...AAV-EF1a-jGCaMP8m-WPRE EF1a GCaMP8m Calcium sensor Looger 176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium...AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f Calcium sensor Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m Calcium...

  6. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...gfaABC1D-lck-GCaMP6f Biosensor Baljit Khakh AV-5-PV3435 100834-AAV5 pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 Biosensor...AV-1-50942 50942-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...AV-1-50943 50943-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...68720 68720-AAV1 pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...

  7. Brain Initiative Collection

    Type
    Collection
    ...158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...element Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons... Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...111261-AAV9 pAAV-hSynapsin1-axon-GCaMP6m For enriched expression of GCaMP6m in axons Lin Tian 111262-AAV1...AAV1 pAAV-hSynapsin1-axon-GCaMP6s For enriched expression of GCaMP6s in axons Lin Tian 111568-AAV5 pZac2.1...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... β1 (white) and anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG...that your target protein is expressed in the hippocampus, or counting the number of puncta in each cell...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2 Hippocampus AAV9>AAV5>AAV8>AAV1>AAV6>AAV2 Auditory Cortex...upon which section you’re looking at (striatum, hippocampus and cortex) within the mouse brain (4). An example...

  11. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical Institute. Today's...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. His interests...

  12. Caltech Systemic Capsids

    Type
    Collection
    ...Cre-dependent GCaMP Kim , GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488... pGP-AAV-syn-jGCaMP7f-WPRE Syn Calcium sensor GCaMP Kim , GENIE 135632 pAAV-S5E2-GCaMP6f E2 regulatory...104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE... Kim , GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn Calcium sensor GCaMP GENIE Optogenetics 26973 pAAV-hSyn-hChR2...regulatory element Calcium sensor GCaMP Dimidschstein 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...irreversibly cleaved when exposed to light. Robert Campbell’s lab illustrates the utility of PhoCl in a variety...Addgene’s FP collection. Just last month, Robert Campbell’s lab added two new mCherry variants to the repository...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...137119 pAAV-EF1a-Con/Fon-GCaMP6M Cre AND Flp 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M Cre AND NOT Flp FRT/F5...137121 pAAV-Ef1a-Coff/Fon-GCaMP6M Flp AND NOT Cre 137122 pAAV-Ef1a-Con/Fon-GCaMP6F Cre AND Flp 137123 pAAV-Ef1a-Con...-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT Flp FRT/F5 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F Flp AND NOT Cre 137125... AND Flp AND Vcre 137165 pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre References Fenno LE, Mattis...

  15. AAVED

    Type
    Collection
    ...Gowan Tervo Janelia Research Campus Sarada Viswanathan Janelia Research Campus Kenton Woodard Penn Vector...materials to share: In vivo data: Retrograde hSyn1-GCaMP6f-P2A-nls-dTomato Detailed protocol: AAV Production... Tervo and Sarada Viswanathan, Janelia Research Campus Kenton Woodard, Penn Vector Core Intended Outcome...

  16. Brain Armamentarium

    Type
    Collection
    ...-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4361) Expression of jGCaMP8s in striatal indirect ...-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) Expression of jGCaMP8s in striatal direct pathway...

  17. Optogenetics AAV Preps

    Type
    Collection
    ...pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive...174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn ChrimsonR (soma-targeted) GCaMP8s Cre dependent 9 Histed...

  18. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...traditions is our annual Twelve Days of CRISPR campaign! But if you missed our tweets featuring the best...

  19. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...Lab from University of Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a case of holiday cheer, and...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...HP10347, L-SIGN, LSIGN, MGC129964, MGC47866 CREB1 cAMP responsive element binding protein 1 CREB, MGC9284...complement component 5a receptor 1 C5A, C5AR, C5R1, CD88 CAMP cathelicidin antimicrobial peptide CAP18, CRAMP,...CTR1 CALCRL calcitonin receptor-like CGRPR, CRLR CAMP cathelicidin antimicrobial peptide CAP18, CRAMP,...
Showing: 41 - 60 of 105 results