...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...Supplementing cell growth media with glucose decreases cAMP and represses pBad, decreasing promoter leakiness...

...in 2010 for visualizing the transcription factor cAMP-responsive element-binding protein (CREB) in living...were then refined by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors...

...previous publications. They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing ...

...pathways HDR mechanisms can be divided into two main camps: non-conservative and conservative. The conservative...

.... "Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and...

...There are three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but...zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors are also suitable for in vivo...slower). GCaMPf may be suitable for very rapid measurements, like action potentials in neurons. jGCaMP7 and...localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif...axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and...to have slower, but larger, calcium changes. The GCaMP6 family of sensors are commonly used calcium reporters...and jGCaMP8 are also available with improved kinetic and sensitivity parameters. Sensitivity and Localization...

...requests faster than jGCaMP7 (Figure 5B).   Figure 5: A) GCaMP6, jGCaMP7, and jGCaMP8 viral preps distributed...Figure 5A). We then added jGCaMP7 and eventually jGCaMP8, later generations of GCaMP6, to the service. While...) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year from 2016–2023. All three...generations: jGCaMP8 allows for the “picture” of a train of action potentials that GCaMP6 and jGCaMP7 do not... for instance, the GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator...they were both requested, GCaMP6 remains the more requested option, and jGCaMP8 appears to be growing in...be a matter of timing, as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of the...

...-fold brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest...pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-jGCaMP7c variant...potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence, as the GCaMP7f variant...potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all our biosensors inventory, and check...Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE (104489-AAVrg) pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics...sensors The latest calcium sensors from Janelia (jGCaMP7) continue to be useful for monitoring neuronal ...

...Flp-dependent GCaMP, AAV8-105714. Several new options are also available for the calcium sensor jGCaMP7 from Janelia... Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most ...104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE 104493 AAVrg pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium...inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.F102G...calcium binding. There are a few versions of these jGCaMP7 sensors with different properties (for example,...applications. We recently released a new variant of jGCaMP7 (keep reading) and will be releasing more variants...like dendrites and axons. Find the 7b variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV ...Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity...Purkinje neurons. 105321  AAV1  pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between...

...axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3...AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors...sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1) pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)   There are hundreds...dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-...

...  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262...AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) 112010  AAV9... pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics ...AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic...Among other new biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast...AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  ...

...repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes, as well as seven...Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-WPRE  AAV9 Biosensor...serotype AAV-hSyn-Soma-jGCaMP8s AAV9 Biosensor Fyhn New serotype AAV-hSyn-Soma-jGCaMP8f AAV9 Biosensor Fyhn...

...logistical stress that accompanies traveling for campus visits. This is definitely an advantage of surviving...everything you need to during the interview. (Virtual) Campus Visits After receiving applications, universities...students to continue the application process through a campus visit, a weekend where applications can get to ...allow you to get important information about the campus even if you can’t visit. By talking with people...aspects that are confusing? During a traditional campus visit, many students will have questions about ... be your perfect fit! Even if you had planned a campus visit and the in-person portion got canceled, taking...And even if you intend to attend an interview or campus visit in person, things like sickness or scheduling...

... Axon targeting GCaMP6f from Lin Tian’s lab is available in serotype AAV1. The GCaMP8 viral vectors from...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ... have arrived! We now offer several versions of GCaMP8 vectors in serotypes AAV1 and AAV5. AAV9 preps ...viral prep in serotype AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in ...ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing...

...regions. For example, communication between the hippocampus and the prefrontal cortex brain regions is involved...specific example of episodic memory, a subset of hippocampal neurons projects axons directly to the prefrontal...Cre recombinase only in pyramidal cells of the hippocampal region CA2 allowed for detailed mapping of the...approach was used by researchers from the Janelia Campus for their MouseLight project but neurons were traced...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2-retro, ...PMC2293646. Jin, J., and Maren, S. (2015). Prefrontal-Hippocampal Interactions in Memory and Emotion. Front Syst...type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits. Nat Neurosci 17(2), 269–279. PubMed...

..., Vibrio cholerae, Xanthomonas campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis...Shewanella spp., Thiobacillus spp., Xanthomonas campestris IncQ RSF1010, R300B, R1162 Gram-negative...salmonicida, Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia IncW pSa, pR388 Gram-...Salmonella typhimurium, Vibrio cholerae, Xanthomonas campestris   In addition to incorporating broad host...

...protein-based calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are optimized for excitation... sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted...axon-targeted syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype ...mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. ...

...which is limited to the hippocampus. Thus, only neurons signaling from the hippocampus to Broca’s area would...poor scalability.  Scientists at Janelia Research Campus and the University of California, Berkeley engineered...access all the neurons that signaled from the hippocampus to Broca’s area. This could be done by injecting...of a mouse specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access ...

...technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where he works on developing and optimizing... anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged 14 days post-transfection...completed, Hubbard will image these neurons using GCaMP7, measuring calcium signaling related fluorescence... the screen to understand mouse cortical and hippocampal neural microcircuits. “In the longer term, this...