...oligonucleotides
Cas9, photocaged-gRNA
Gene editing
Manna et al., 2019
NPPOC-caged trimethoprim (TMP)
...transcription
Zhou et al., 2020
NPOM-caged nucleobases
Cas9, photocaged-gRNA
Gene editing
For more details...chromatin, and study kinetics.
Using chemistry to photocage CRISPRs
The aforementioned techniques each employed...These researchers used a genetically encoded photocaging technique to insert a light-removable protecting...protecting group, specifically a nitrobenzyl photocaged lysine (PCK), on the Cas9 protein (Hemphill et al.,..., read this article).
The group first tested photocaging various lysines in Cas9 to determine which best... ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen in the figure below....
