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Showing: 41 - 60 of 100 results

  1. Chemogenetics AAV Preps

    Type
    Collection
    ...GlyR Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element Tag Fusion tags mCherry HA ...mCherry fusion none 1, 2, 5, 8, 9, rg* Roth 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP...

  2. Mammalian RNAi Tools

    Type
    Collection
    ... 11643 pLVCT-tTR-KRAB 2nd generation; Transgene (CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned... pLVCT-rtTR-KRAB-2SM2 2nd generation; Transgene (CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned...

  3. Viral Production

    Type
    Collection
    ...AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) ...Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward...

  4. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...PV2509 29777-AAV5 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-...105554-AAV1 pENN.AAV.CAG.tdTomato.WPRE.SV40 James M. Wilson AV-1-PV2509 29777-AAV1 pAAV-CAG-ArchT-GFP Ed...Huebener, Tobias Rose AV-1-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer...Huebener, Tobias Rose AV-1-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Tobias Bonhoeffer...ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE...98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40 Loren Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato Hongkui...Loren Looger AV-1-PV2725 98932-AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 Loren Looger AV-1-PV2816 100835-AAV1...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Friedreich ataxia Michael Ristow 14994 pDRIVE-CAG-hFX-HA FXN HA, AU1 CAG Friedreich ataxia Michael Ristow 15239...wtTDP43tdTOMATOHA TARDBP HA, tdTomato CAG ALS Zuoshang Xu 28206 TDP43 NOTAG1 TARDBP CAG ALS Zuoshang Xu 28207 TDP43...TDP43 NOTAG2 TARDBP CAG ALS Zuoshang Xu 28208 TDP43 NOTAG3 TARDBP CAG ALS Zuoshang Xu 28209 TDP43 NOTAG6...NOTAG6 TARDBP CAG ALS Zuoshang Xu 28210 TDP43 NOTAG11 TARDBP CAG ALS Zuoshang Xu 29340 pcDNA3.1/GS-DJ1-R98Q-V5...30137 pCAX APP 695 APP CAG Alzheimer's Dennis Selkoe 30138 pCAX APP 751 APP CAG Alzheimer's Dennis Selkoe...pCAX APP delta CT APP CAG Alzheimer's Dennis Selkoe 30144 pCAX APP AENATA APP CAG Alzheimer's Dennis Selkoe...30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP C99 APP CAG Alzheimer's Dennis Selkoe...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...that AAV-PHP.eB, when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory...is quite pronounced, even when using a ubiquitous CAG promoter. Spatial transcriptomics shows that its ...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...MERS, CoV-1, CoV-2), modification, or variant. pBOB-CAG-SARS-CoV2-Spike-HA (Addgene plasmid #141347) is available...

  9. Lentiviral Prep Service

    Type
    Collection
    ...Expression of SARS-CoV-2 orf3a protein Krogan 141347 pBOB-CAG-SARS-CoV2-Spike-HA Expression of SARS-CoV-2 spike...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Autobow systems are available with either the Thy1 or CAG promoter, while Brainbow AAV is under control of ...target sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...

  13. Promoters

    Type
    Guide
    ...mammalian promoter from human elongation factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter PGK...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...mice. The Jacks Lab used this backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus...pCS2TAL3-RR Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination...pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1...

  15. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...oligonucleotides Cas9, photocaged-gRNA Gene editing Manna et al., 2019   NPPOC-caged trimethoprim (TMP) ...transcription Zhou et al., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA Gene editing For more details...chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques each employed...These researchers used a genetically encoded photocaging technique to insert a light-removable protecting...protecting group, specifically a nitrobenzyl photocaged lysine (PCK), on the Cas9 protein (Hemphill et al.,..., read this article). The group first tested photocaging various lysines in Cas9 to determine which best... ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen in the figure below....

  16. Chemogenetics Guide

    Type
    Guide
    ... GFAP Glia CD68 Microglia Dlx Interneurons EF1a, CAG General expression Localization. Depending on the...

  17. Plasmid Cloning by PCR

    Type
    Blog Post
    ...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...
Showing: 41 - 60 of 100 results